The effects of sodium tanshinone ⅡA sulfonate pretreatment on high glucose-induced fractalkine expression in human umbilical vein endothelial cells

ZHOU Ping; ZENG De-kang; LI Fa-qi; ZHANG Bing; YIN Ling

doi:10.13406/j.cnki.cyxb.2011.07.006

您当前的位置：

首页 >

文章列表页 >

The effects of sodium tanshinone ⅡA sulfonate pretreatment on high glucose-induced fractalkine expression in human umbilical vein endothelial cells

更新时间：2024-01-17

- The effects of sodium tanshinone ⅡA sulfonate pretreatment on high glucose-induced fractalkine expression in human umbilical vein endothelial cells
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 793-796(2011)
- 作者机构：
  
  重庆医科大学附属第一医院老年病科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.07.006
  CLC： R285.5
- Published：2011，
扫描看全文
[1]周平,曾得康,李法琦,张彬,殷菱.丹参酮ⅡA磺酸钠预处理对高糖诱导的人脐静脉内皮细胞fractalkine表达的影响[J].重庆医科大学学报,2011,36(07):793-796.

ZHOU Ping, ZENG De-kang, LI Fa-qi, et al. The effects of sodium tanshinone ⅡA sulfonate pretreatment on high glucose-induced fractalkine expression in human umbilical vein endothelial cells. [J]. Journal of Chongqing Medical University 36(7):793-796(2011)
[1]周平,曾得康,李法琦,张彬,殷菱.丹参酮ⅡA磺酸钠预处理对高糖诱导的人脐静脉内皮细胞fractalkine表达的影响[J].重庆医科大学学报,2011,36(07):793-796. DOI： 10.13406/j.cnki.cyxb.2011.07.006.

ZHOU Ping, ZENG De-kang, LI Fa-qi, et al. The effects of sodium tanshinone ⅡA sulfonate pretreatment on high glucose-induced fractalkine expression in human umbilical vein endothelial cells. [J]. Journal of Chongqing Medical University 36(7):793-796(2011) DOI： 10.13406/j.cnki.cyxb.2011.07.006.

摘要

目的:探讨丹参酮ⅡA磺酸钠（Sodium tanshinoneⅡA sulfonate

STS）预处理对高糖诱导的人脐静脉内皮细胞（Humanumbilical vein endothelial cells

HUVECs）fractalkine（FKN）表达的影响及其可能机制。方法:对数生长期的HUVECs

分为正常组（N）

高糖组（HG）

STS组（STS）

高糖加STS组（HG+STS）

高糖加STS与氯化锂组（HG+STS+LiCl）

5.5 mm的低糖DMEM培养的HUVECs种板10 h后按以上分组加入5 mg/L的STS和（或）10 mm的LiCl预处理2 h

再用33.3 mm的高糖干预48 h后

免疫组化SABC法检测β-catenin及p-GSK-3β（Ser9）、免疫荧光FITC法检测FKN蛋白水平变化

RT-PCR检测GSK-3β和FKNmRNA水平变化。结果:与正常组比较

高糖组FKN蛋白及mRNA水平（P<0.05）

GSK3βmRNA水平（P<0.05）明显增加

β-catenin及p-GSK-3β（Ser9）蛋白表达（P<0.05）降低;与高糖组比较

STS组FKN蛋白及mRNA水平（P<0.05）

GSK3βmRNA水平（P<0.05）明显降低

但仍高于正常组

β-catenin及p-GSK-3β（Ser9）蛋白表达（P<0.05）增加

但仍低于正常组;STS与氯化锂共同预处理后以上改变更明显（P<0.05）。结论:STS抑制HUVEC FKN表达可能与Wnt信号通路有关。

Abstract

Objective:To explore the effects of sodium tanshinone ⅡA sulfonate(STS) pretreatment on high glucose-induced expression of fractalkine(FKN) in human umbilical vein endothelial cells(HUVECs) and its possible mechanisms.Methods:HUVECs in good condition were divided into 5 groups:normal group(N)

high glucose group(HG)

STS group(STS)

high glucose with STS group(HG+STS) and high glucose with STS and lithium chloride group(HG+STS+LiCl).After planted HUVECs in DMEM/LG(5.5 mmol/L) for 10 h

5 mg/L STS and/or 10mmol/L LiCl was added for 2 h as above and then 33.3 mmol/L high glucose was added for 48h.Harvested and the protein 1evels of intracellular β-catenin and p-GSK-3β(Ser9) were measured by immunohistochemistry staining(SABC)and membrane fractalkine were detected by immunofluorescence(FITC)

and the mRNA expression of GSK-3β and FKN were measured by RT-PCR.Results:Compare with N

the mRNA levels of GSK-3β(P<0.05) and both the protein and mRNA level(P<0.05) of fracalkine were increased and the protein expression of β-catenin and p-GSK-3β(Ser9)(P<0.05) were decreased in HG;Compare with HG

the mRNA levels of GSK-3β(P<0.05) and both the protein and mRNA levels(P<0.05) of fractalkine were decreased and the protein levels of β-catenin and p-GSK-3β(Ser9) were improved(P<0.05) in HG+STS;and both STS and lithium chloride pretreatment enhanced the effects above(P<0.05).Conclusion:STS pretreatment suppress the expression of FKN in HUVEC induced by high glucose and it may be relevant to Wnt signaling pathway.

关键词

丹参酮ⅡA磺酸钠高糖fractalkine人脐静脉内皮细胞Wnt信号通路

Keywords

sodium tanshinone ⅡA sulfonatehigh glucosefractalkinehuman umbilical vein endothelial cellsWnt signaling pathway

references

Views

111

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Ezhu-containing serum inhibiting the expression of Gli1 and β-catenin in hepatic stellate cells

Effects of HIF1α-KIM1 signaling pathway on renal interstitial fibrosis in diabetic nephropathy

Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells

The influence of slidroside to wnt signaling pathway in rat hepatic stellate cells stimulated by acetaldehyde

Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose

Related Author

Ye Feng

Cao Wenfu

Feng Lili

Dang Yong

Li Yaqian

Yu Fang

胡长林

et al

Related Institution

Department of Integration of Traditional Chinese Medicine and Western Medicine,The First Affiliated Hospital of Chongqing Medical University

Department of Integration of Traditional Chinese Medicine and Western Medicine,People’s Hospital of Changshou of Chongqing

Department of Nephrology,Anyang People’s Hospital Henananyang

The First Clinical Medical College of Nanchang University

Department of Endocrinology,the Second Affiliated Hospital,Chongqing Medical University

⁰