Effect of the cadmium on the expression of vimentin &amp; E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats

LIAO Xiao-gang; SHI Zhi-hu; ZOU Cong; MAO Sheng-yan; FAN Jing-chuan

doi:10.13406/j.cnki.cyxb.2011.07.004

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Effect of the cadmium on the expression of vimentin & E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats

更新时间：2024-01-17

- Effect of the cadmium on the expression of vimentin & E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 814-817(2011)
- 作者机构：
  
  重庆医科大学生命科学研究院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.07.004
  CLC： R114
- Published：2011，
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[1]廖晓岗,石之虎,邹聪,毛胜艳,范京川.镉对大鼠睾丸支持细胞波形蛋白、E-钙黏蛋白表达及血睾屏障的影响[J].重庆医科大学学报,2011,36(07):814-817.

LIAO Xiao-gang, SHI Zhi-hu, ZOU Cong, et al. Effect of the cadmium on the expression of vimentin & E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats. [J]. Journal of Chongqing Medical University 36(7):814-817(2011)
[1]廖晓岗,石之虎,邹聪,毛胜艳,范京川.镉对大鼠睾丸支持细胞波形蛋白、E-钙黏蛋白表达及血睾屏障的影响[J].重庆医科大学学报,2011,36(07):814-817. DOI： 10.13406/j.cnki.cyxb.2011.07.004.

LIAO Xiao-gang, SHI Zhi-hu, ZOU Cong, et al. Effect of the cadmium on the expression of vimentin & E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats. [J]. Journal of Chongqing Medical University 36(7):814-817(2011) DOI： 10.13406/j.cnki.cyxb.2011.07.004.

摘要

目的:探讨镉（Cadmium）对大鼠睾丸支持细胞波形蛋白、E-钙黏蛋白表达和血睾屏障的影响。方法:21只6周龄雄性SD大鼠随机分为单纯镉组（0.1%氯化镉腹腔内注射

1 mg/（kg.bw）

每周连续注射5 d

处理后1、2、3、4周取材）和对照组（腹腔内注射等量生理盐水）;镧示踪组和镉加镧组（镉剂量同上

1周取材）

各实验组及对照组均为3只动物。取睾丸作光镜免疫组化和图像分析、血睾屏障超微结构及镧示踪观察。结果:波形蛋白阳性产物主要在支持细胞近基室腔的胞浆中表达

并向曲细精管管腔呈辐射状延伸;E-钙黏蛋白阳性产物主要定位于生精上皮近腔室的支持细胞和部分生精细胞胞浆中。镉处理后两种蛋白阳性产物表达的平均光密度值明显降低

支持细胞胞质特化区和紧密连接破坏。镉加镧处理组硝酸镧透过支持细胞紧密连接间隙。结论:镉减弱大鼠睾丸支持细胞波形蛋白和E-钙黏蛋白的表达

造成支持细胞血睾屏障的损伤。

Abstract

Objective:To investigate the effect of the cadmium(Cd)on the expression of vimentin(V)& E-cadherin(E-cad)in Sertoli cell(Sc)and the ultrastructure of blood-testis barrier(BTB)in rats.Methods:21 SD male rats were randomly divided into Cd group(rats of different interval from 1 w to 4 w time were intraperitoneally injected with 0.1% CdCl2

1 mg/(kg·bw)

5 days a week)and control group(rats were intraperitoneally injected with equal dosage of normal saline);lanthanum nitrate(La)tracing group and Cd+La group.The testes were respectively studied by light

electron microscope and immunohistochemistry of V & E-cad and by semi-quanitatively image analysis.Results:The positive product of V was expressed in cytoplasm of Sc

and elongated radially to lumens in seminiferous tubules.But

the positive product of E-cad was localized mainly in cytoplasm of Sc and some spermatogenic cells.After Cd treatment

the average optical density(AOD) of V and E-cad were decreased obviously;the ultrastructure damage of BTB and ectoplasmic specializations(Es)in Sc were observed.The La-tracing showed that tight junction(TJ)of Sc was infiltrated by La in Cd+La group.Conclusion:Cd decreases the expression of V and E-cad in Sc and damages BTB of Sc in rats.

关键词

镉支持细胞硝酸镧波形蛋白E-钙黏蛋白

Keywords

cadmiumsertoli cellslanthanum nitrateVimentinE-cadherin

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Effect of the cadmium on the expression of vimentin & E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats

DOI：10.13406/j.cnki.cyxb.2011.07.004

摘要

Abstract

关键词

Keywords

references