Expression of NSA2 in a mouse model of choroidal neovascularization

CHEN Xin-ke; PENG Hui; LEI Bo; WANG Juan; FU Peng

doi:10.13406/j.cnki.cyxb.2011.06.035

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Expression of NSA2 in a mouse model of choroidal neovascularization

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- Expression of NSA2 in a mouse model of choroidal neovascularization
- Journal of Chongqing Medical University Vol. 36, Issue 6, Pages: 704-706(2011)
- 作者机构：
  
  重庆医科大学附属第一医院眼科眼科学重庆市市级重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.06.035
  CLC： R773.4
- Published：2011，
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[1]陈新科,彭惠,雷博,王隽,付鹏.核蛋白NSA2在脉络膜新生血管动物模型眼组织的表达研究[J].重庆医科大学学报,2011,36(06):704-706.

CHEN Xin-ke, PENG Hui, LEI Bo, et al. Expression of NSA2 in a mouse model of choroidal neovascularization. [J]. Journal of Chongqing Medical University 36(6):704-706(2011)
[1]陈新科,彭惠,雷博,王隽,付鹏.核蛋白NSA2在脉络膜新生血管动物模型眼组织的表达研究[J].重庆医科大学学报,2011,36(06):704-706. DOI： 10.13406/j.cnki.cyxb.2011.06.035.

CHEN Xin-ke, PENG Hui, LEI Bo, et al. Expression of NSA2 in a mouse model of choroidal neovascularization. [J]. Journal of Chongqing Medical University 36(6):704-706(2011) DOI： 10.13406/j.cnki.cyxb.2011.06.035.

摘要

目的:NSA2是1种新发现的与细胞增殖相关的核蛋白

本实验旨在初步探讨NSA2是否参与了脉络膜新生血管（Chor-oidal neovascularization

CNV）病变过程。方法:采用532 nm波长的眼底激光对16只小鼠光凝处理

诱导CNV动物模型;小鼠眼底激光光凝处理5 d后

去除眼前段

定量PCR检测NSA2 mRNA;眼球冰冻切片

用NSA2抗体对NSA2蛋白进行间接免疫荧光染色。结果:相对于未经过任何处理的正常对照组

CNV组NSA2 mRNA表达明显上调（P<0.05）。较正常脉络膜组织

CNV组织细胞NSA2阳性率明显增高（P<0.05）;CNV区域附近的节细胞NSA2蛋白检测呈阳性

与之相对的正常对照组和CNV组中的远离CNV区域

均未发现NSA2阳性表达的节细胞。结论:NSA2在CNV动物模型眼组织中表达上调

我们推测NSA2可能与CNV病变过程相关联。

Abstract

Objective: NSA2

a newly documented nuclear protein

plays an important role in cell proliferation.We investigated whether NSA2 is involved in the choroidal neovascularization(CNV)

a common pathological process in many retinal diseases.Methods: CNV model was induced by 532 nm laser coagulation in 16 mice.Five days after laser photocoagulation

NSA2 mRNA level in the posterior segment of the eyes was detected by Real-time quantitative PCR.NSA2 expression in eyeballs was detected by immunofluorescence staining with a NSA2 antibody on frozen sections.Results:Compared with the untreated control group

the level of NSA2 mRNA was scientifically up-regulated in the CNV group(P<0.05).Immunofluorescence showed that the rate of NSA2-positive cell in CNV tissue was higher than that in normal choroidal tissue(P<0.05);NSA2-positive ganglion cells were found near the CNV area in the treated eyes

but was not in the remote area of the CNV eyes and in the control eyes.Conclusion:We found that NSA2 was up-regulated in with a mouse model of CNV.Our data suggested that the nuclear protein may associate with the pathology of choroidal neovascularization.

关键词

脉络膜新生血管动物模型核蛋白

Keywords

choroidal neovascularizationanimal modelnucleoprotein

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