Influence of shRNA interfering with PARG on the expression of cell adhesion fact

YAN Jia-xin; WANG Ya-lan; WU Wei-qiang; PAN Juan

doi:10.13406/j.cnki.cyxb.2011.06.029

您当前的位置：

首页 >

文章列表页 >

Influence of shRNA interfering with PARG on the expression of cell adhesion fact

更新时间：2024-01-17

- Influence of shRNA interfering with PARG on the expression of cell adhesion fact
- Journal of Chongqing Medical University Vol. 36, Issue 6, Pages: 641-645(2011)
- 作者机构：
  
  重庆医科大学基础医学院病理学教研室分子医学与肿瘤研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.06.029
  CLC： R735.34
- Published：2011，
扫描看全文
[1]颜佳欣,王娅兰,吴伟强,潘娟.shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响[J].重庆医科大学学报,2011,36(06):641-645.

YAN Jia-xin, WANG Ya-lan, WU Wei-qiang, et al. Influence of shRNA interfering with PARG on the expression of cell adhesion fact. [J]. Journal of Chongqing Medical University 36(6):641-645(2011)
[1]颜佳欣,王娅兰,吴伟强,潘娟.shRNA干扰PARG对大肠癌CT26细胞粘附因子表达的影响[J].重庆医科大学学报,2011,36(06):641-645. DOI： 10.13406/j.cnki.cyxb.2011.06.029.

YAN Jia-xin, WANG Ya-lan, WU Wei-qiang, et al. Influence of shRNA interfering with PARG on the expression of cell adhesion fact. [J]. Journal of Chongqing Medical University 36(6):641-645(2011) DOI： 10.13406/j.cnki.cyxb.2011.06.029.

摘要

目的:探讨小鼠大肠癌CT26株中聚（腺苷二磷酸核糖）水解酶[Poly（ADP-ribose）glycohydrolase

PARG]对细胞间粘附因子表达的影响及其可能的机制。方法:以慢病毒为载体对CT26细胞株中PARG基因进行RNA沉默干扰

并筛选出稳定表达株。Realtime-PCR法检测沉默效果。Western blot法检测CT26细胞PARG、PARP、NF-κB和Pi-AKT（473）I、CAM-1、P-selectin的蛋白表达。结果:实验组与对照组（包括阴性对照组、空载体对照组）相比

PARG、PARP、Pi-AKT（473）、NF-κBI、CAM-1、P-selectin表达经统计学分析差异均有统计学意义（P<0.05）。结论:在大肠癌细胞株中

抑制PARG可以通过调节与NF-κB有关通路

进一步调节ICAM-1、P-selectin等细胞粘附因子。提示PARG在肿瘤癌栓形成过程中可能具有重要作用。

Abstract

Objective:To clarify the possible mechanism of how PARG and PARP are to regulate intercellular adhesion factor through relevant signal pathways.Methods:Lentivirus carried shRNA interfered with the expression of PARG and steadily silenced cell lines.Realtime-PCR was used for testing the strength of shRNAi and Western blot was used to test the expressions of PARG

PARP

NF-κB

and Pi-AKT(473)

and LY249002 was served as Pi-AKT inhibitor.Results:The expressions of PARG

PARP

and NF-κB

ICAM-1

P-selectin in shRNAi group were weaker than those in the control groups(blank control and transfected with empty vector control group);whereas Pi-AKT increased in the shRNAi group(P<0.05).Conclusion:The results suggest that PARG gene silencing may regulate NF-κB related signaling pathway and the intercellular adhesion factors such as ICAM-1 and P-selectin

which are reduced.

关键词

PARG慢病毒转染AKT磷酸化细胞粘附

Keywords

poly(ADP-ribose)glycohydrolaselentiviral transfectionAKT phosphorylationcell adhesion

references

Views

112

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The effect of BCG - PSN on the mechanical properties of adhesion between PG cells or PAa cells and HUVEC

Effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of gastric cancer cells

The relation between poly(ADP-ribose) glycohydrolase,poly(ADP-ribose) polymerase and AKT phosphorylation in colorectal carcinoma cell

Influence of shRNA claudin-1 on invasion and metastasis of SW620 cells

Related Author

SHEN Qing

et al

吴泽志

LUO Jun

et al

LI Qiao-zhuan

et al

李娴

Related Institution

Department of Gerontology, the Third People ’ s Hospital of Chongqing

重庆医科大学第二临床学院呼吸内科

重庆大学生物工程学院

Department of Digestion,Central Hospital of Nanchong

⁰