Effects of puerarin on learning and memory and NMDAR in rats following hypoxic-ischemic brain damage

SHENG Zhi-qiang; XIAO Nong; XU Bei; ZHANG Xiao-ping

doi:10.13406/j.cnki.cyxb.2011.06.003

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Effects of puerarin on learning and memory and NMDAR in rats following hypoxic-ischemic brain damage

更新时间：2024-01-17

- Effects of puerarin on learning and memory and NMDAR in rats following hypoxic-ischemic brain damage
- Journal of Chongqing Medical University Vol. 36, Issue 6, Pages: 663-666(2011)
- 作者机构：
  
  重庆医科大学附属儿童医院康复中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.06.003
  CLC： R285.5
- Published：2011，
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[1]盛志强,肖农,许蓓,张晓萍.葛根素对缺氧缺血脑损伤后学习记忆能力及NMDA受体的影响[J].重庆医科大学学报,2011,36(06):663-666.

SHENG Zhi-qiang, XIAO Nong, XU Bei, et al. Effects of puerarin on learning and memory and NMDAR in rats following hypoxic-ischemic brain damage. [J]. Journal of Chongqing Medical University 36(6):663-666(2011)
[1]盛志强,肖农,许蓓,张晓萍.葛根素对缺氧缺血脑损伤后学习记忆能力及NMDA受体的影响[J].重庆医科大学学报,2011,36(06):663-666. DOI： 10.13406/j.cnki.cyxb.2011.06.003.

SHENG Zhi-qiang, XIAO Nong, XU Bei, et al. Effects of puerarin on learning and memory and NMDAR in rats following hypoxic-ischemic brain damage. [J]. Journal of Chongqing Medical University 36(6):663-666(2011) DOI： 10.13406/j.cnki.cyxb.2011.06.003.

摘要

目的:观察葛根素（Puerarin

Pue）对新生鼠缺氧缺血后学习记忆的远期影响及其可能机制。方法:7 d龄SD大鼠随机分为3组:假手术组（Sham组）;对照组（Con组）;葛根素组（Pue组）。各组分别于8周龄时Morris水迷宫检测其学习记忆能力

于3、4周和8周龄时采用免疫组化方法检测各组NR2A和NR2B在海马CA1区的表达。结果:①8周时Con组比Sham组平均潜伏期明显延长（P<0.05）

穿越平台次数显著减少（P<0.01）;与Con组相比

Pue组的平均潜伏期和穿越平台次数均得到明显改善（P<0.05）。②Con组3、4周和8周时海马CA1区NR2A和NR2B的表达均明显低于Sham组（P<0.01）;Pue组NR2A蛋白在3周和8周时的表达比同一时间点Con组明显增多（P<0.05）

NR2B蛋白在3周和4周时的表达明显比Con组增多（P<0.05）。结论:Pue能提高缺氧缺血新生大鼠年长后的学习记忆能力

其保护作用可能和Pue增加海马CA1区NR2A和NR2B的表达有关。

Abstract

Objective:To investigate the effects of puerarin on learning and memory ability after hypoxia-ischemia brain damage and to understand the underlying mechanisms.Methods:A total of 102 S-D rats(P7)were randomly assigned to three groups(n=34):sham group

Con group

and puerarin group.At 8 weeks after birth

learning and memory ability were tested by Morris water maze.At 3

8 weeks after birth

expressions of NR2A and NR2B were detected in the CA1 region of the hippocampus by immunohistochemistry.Results:①The average time of escape latency was significantly longer and the number of platform location crossing was less in Con group than these in sham group(P<0.05

P<0.01).Compared with Con group

the average time of escape latency was significantly shorter(P<0.05)and the number of platform location crossing was more(P<0.05)in puerarin group.②The expression levels of NR2A and NR2B in hippocampal CA1 areas in the Con group were less than those in the sham-operated rats at any time points(P<0.01).These levels of NR2A were significantly increased in the puerarin group when the rats were 3 w and 8 w of age

compared with the Con group(P<0.05).These levels of NR2B were significantly increased in the puerarin group when the rats were 3 w and 4 w of age

compared with the Con group(P<0.05).Conclusion:Puerarin can improve learning and memory in hypoxia-ischemia brain damage rats.This protection may be associated with the increased NR2A and NR2B in the CA1 region of hippocampus.

关键词

葛根素缺氧-缺血N-甲基-D-天冬氨酸受体学习记忆

Keywords

Puerarinhypoxic-ischemicN-methyl-D-aspartate receptorlearning and memory

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