Objective:To discuss the effect of achyranthes bidentata polysaccharide and its combination with 5-fluorineuracils on esophagus cancer EC9706 cell function and the possible mechanism.Methods:Three different concentration of ABPS100

200

300 μg/ml

and combined with 5-fluorouracil cell multiplication suppression rate was detected through cell strain growth inhibition curve(MTT);the cell cycle change and apoptosis were examined through rate flow cytometry;the effect of achyranthes bidentata polysaccharides on cell cycle related gene p21

p27 expressions of esophagus cancer cell was detected through Western blot;cell apoptosis related gene bax and bcl-2expression were detected through immunohistochemistry.Results:1)The acetyltalatisamine achyranthan could suppress esophagus cancer EC9706 cell’s multiplication and proliferation;the killing action to esophagus cancer cell was stronger when combined with 5-fluorouracil than when they were used separately.2)After using achyranthes bidentata polysaccharides

the esophagus cancer EC9706 was blocked in G0/G1 phase

and the cells expressed the early time apoptosis.Its mechanism possibly involves the increase of p21 and p27 gene expressions separately as well as the increase of bax gene expression with the bcl-2 gene expression reduction.Conclusion:The achyranthes bidentata polysaccharides has the inhibitory action to the esophagus cancer cell multiplication;the acetyltalatisamine achyranthan has the anti-tumor function with 5-fluorouracil.