Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells

SUN Di; SHEN Yi; XIE Ying-shan; LONG Shuang

doi:10.13406/j.cnki.cyxb.2011.05.025

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Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells

更新时间：2024-01-17

- Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells
- Journal of Chongqing Medical University Vol. 36, Issue 5, Pages: 520-523(2011)
- 作者机构：
  
  重庆医科大学基础医学院干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.05.025
  CLC： R737.33
- Published：2011，
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[1]孙迪,沈宜,谢莹珊,隆霜.宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究[J].重庆医科大学学报,2011,36(05):520-523.

SUN Di, SHEN Yi, XIE Ying-shan, et al. Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells. [J]. Journal of Chongqing Medical University 36(5):520-523(2011)
[1]孙迪,沈宜,谢莹珊,隆霜.宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究[J].重庆医科大学学报,2011,36(05):520-523. DOI： 10.13406/j.cnki.cyxb.2011.05.025.

SUN Di, SHEN Yi, XIE Ying-shan, et al. Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells. [J]. Journal of Chongqing Medical University 36(5):520-523(2011) DOI： 10.13406/j.cnki.cyxb.2011.05.025.

摘要

目的:观察MAPK信号通路阻断剂UO-126对人类宫颈癌细胞株HeLa细胞周期素E（cyclinE）及F-盒和含蛋白7的WD重复结构域FBW7表达的影响。方法:采用RT-PCR、Western blot检测UO-126阻断MAPK信号通路前后cyclin E和FBW7 mRNA及蛋白表达情况。结果:RT-PCR及Western blot结果显示

UO-126作用后HeLa细胞cyclin E mRNA表达水平无明显变化

cyclin E蛋白表达水平明显下降

FBW7 mRNA及蛋白表达水平较未处理HeLa细胞均明显增高。结论:宫颈癌HeLa细胞中FBW7异常表达与MAPK信号通路激活密切相关。UO-126阻断MAPK信号通路可下调cyclin E表达

上调FBW7表达。

Abstract

Objective:To investigate the effects of UO-126

a MAPK inhibitor

on the expression of cyclin E and F-box and WD repeat domain-containing protein 7(FBW7)of human cervical cancer cell line(HeLa cells).Methods:The mRNA and protein expression of cyclin E and FBW7 were detected by RT-PCR and Western blot before and after MAPK signal was blocked by UO-126

a MAPK inhibitor.Results:RT-PCR and Western blot exhibited that cyclin E mRNA expression had no significant changes and cyclin E protein had significantly decreased after HeLa cells were treated with UO-126

and FBW7 mRNA and protein expression had significantly increased after HeLa cells were treated with UO-126.Conclusion:The abnormal expression of FBW7 is closely related to the activation of MAPK signaling pathway in cervical cancer HeLa cells.Cyclin E is decreased and FBW7 is increased after UO-126 blocks the MAPK signaling pathway.

关键词

UO-126MAPK宫颈癌细胞株（HeLa细胞）细胞周期素EFBW7

Keywords

UO-126MAPKcervical cancer HeLa cellscyclin EFBW7

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Department of Cardiology,the Second Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第二医院心内科

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