Expression of TLR4 and NF-κBp50 in renal ischemia-reperfusion injury and their significance

YU Xiao-dong; DENG Xian-zhong; JIANG Guo; LIAO Bo; ZHU Ping-yu; LU Dong-liang; TANG Tie-long; YANG Xue-song; CHEN Shuang-quan; CHENG Shu-lin

doi:10.13406/j.cnki.cyxb.2011.05.023

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Expression of TLR4 and NF-κBp50 in renal ischemia-reperfusion injury and their significance

更新时间：2024-01-17

- Expression of TLR4 and NF-κBp50 in renal ischemia-reperfusion injury and their significance
- Journal of Chongqing Medical University Vol. 36, Issue 5, Pages: 557-559(2011)
- 作者机构：
  
  川北医学院附属医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.05.023
  CLC： R692
- Published：2011，
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[1]余晓东,邓显忠,姜果,廖波,朱平宇,鲁栋梁,唐铁龙,杨雪松,陈双全,程树林.TLR4和NF-κBp50在肾缺血再灌注损伤中的表达及意义[J].重庆医科大学学报,2011,36(05):557-559.

YU Xiao-dong, DENG Xian-zhong, JIANG Guo, et al. Expression of TLR4 and NF-κBp50 in renal ischemia-reperfusion injury and their significance. [J]. Journal of Chongqing Medical University 36(5):557-559(2011)
[1]余晓东,邓显忠,姜果,廖波,朱平宇,鲁栋梁,唐铁龙,杨雪松,陈双全,程树林.TLR4和NF-κBp50在肾缺血再灌注损伤中的表达及意义[J].重庆医科大学学报,2011,36(05):557-559. DOI： 10.13406/j.cnki.cyxb.2011.05.023.

YU Xiao-dong, DENG Xian-zhong, JIANG Guo, et al. Expression of TLR4 and NF-κBp50 in renal ischemia-reperfusion injury and their significance. [J]. Journal of Chongqing Medical University 36(5):557-559(2011) DOI： 10.13406/j.cnki.cyxb.2011.05.023.

摘要

目的:观察大鼠肾缺血再灌注后Toll样受体4（Toll-like receptors 4

TLR4）和细胞核因子kappa B（Nuclear factorkappa B

NF-κB）p50蛋白的表达情况

探讨它们参与肾缺血再灌注损伤的作用机理。方法:SD雄性大鼠48只随机分为对照组（Control组

简称C组）、缺血再灌注组（Ischemia-reperfusion组

简称Ir组）2个实验组

每组各4个时相（6、12、24、72 h）。采用切除右肾

夹闭左肾动脉45 min后恢复灌流建立肾缺血再灌注损伤模型。免疫组化二步法检测大鼠肾组织中TLR4和NF-κBp50蛋白的表达情况

测定髓过氧化物酶（Myeloperoxidase

Mpo）活性及血清肌酐（Serum creatinine

Scr）水平以反映中性粒细胞活化及肾功能损害的程度。结果:TLR4和NF-κBp50蛋白在C组中几乎无表达

Ir组中表达明显增强（P<0.01）。与C组比较

Ir组Scr水平明显升高

Mpo活性显著增加（P<0.01）。结论:肾缺血再灌注损伤的发生可能与TLR4活化NF-κB诱导的炎症反应有关。

Abstract

Objective:To investigate the expression of TLR4 protein and NF-κBp50 protein after renal ischemia-reperfusion and study their significance in renal ischemia-reperfusion injury.Methods:48 normal male Sprague-Dawley rats were randomly divided into two groups:control group(C group)and ischemia-reperfusion group(Ir group).The renal ischemia-reperfusion injury model was established by clamping the left renal artery for 45 min and reperfusion for 6

24 h and 72 h

then the right kidneys were removed before clamping left renal vessels.Two-step immunohistochemical methods were used to detect the changes in the expression of TLR4 protein and NF-κBp50 protein.Myeloperoxidase(Mpo) activity and serum creatinine(Scr) levels were determined

which reflected neutrophil activation and kidney dysfunction.Results:The Mpo activity and the Scr levels were increased remarkably in Ir group

as compared with C group(P<0.01).The expression of TLR4 protein and NF-κBp50 protein were gradually increased with the progress of ischemia-reperfusion(P<0.01).Conclusion:The occurrence of renal ischemia-reperfusion injury may correlate with the inflammatory response induced by TLR4 activating NF-κB.

关键词

Toll样受体Toll样受体4细胞核因子kappa B缺血再灌注损伤

Keywords

Toll-like receptorstoll-like receptor 4nuclear factor kappa Bischemia-reperfusion injury

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