Experimental study of recombinant human endostatin combined with NVB-Cisplatin on human breast cancer xenografts in nude mice

CHENG Li1; ZHANG Xing-ping1; YANG Hong-jie2; ZHOU Tao-qi1; RAO Ming-yue1

doi:10.13406/j.cnki.cyxb.2011.05.018

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Experimental study of recombinant human endostatin combined with NVB-Cisplatin on human breast cancer xenografts in nude mice

更新时间：2024-01-17

- Experimental study of recombinant human endostatin combined with NVB-Cisplatin on human breast cancer xenografts in nude mice
- Journal of Chongqing Medical University Vol. 36, Issue 5, Pages: 546-549(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肿瘤科
  2. 成都医学院基础医学院形态学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.05.018
  CLC： R737.9
- Published：2011，
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[1]程丽,张幸平,杨鸿捷,周涛琪,饶明月.重组人血管内皮抑素联合NP方案对人乳腺癌裸鼠移植瘤作用的实验研究[J].重庆医科大学学报,2011,36(05):546-549.

CHENG Li1, ZHANG Xing-ping1, YANG Hong-jie2, et al. Experimental study of recombinant human endostatin combined with NVB-Cisplatin on human breast cancer xenografts in nude mice. [J]. Journal of Chongqing Medical University 36(5):546-549(2011)
[1]程丽,张幸平,杨鸿捷,周涛琪,饶明月.重组人血管内皮抑素联合NP方案对人乳腺癌裸鼠移植瘤作用的实验研究[J].重庆医科大学学报,2011,36(05):546-549. DOI： 10.13406/j.cnki.cyxb.2011.05.018.

CHENG Li1, ZHANG Xing-ping1, YANG Hong-jie2, et al. Experimental study of recombinant human endostatin combined with NVB-Cisplatin on human breast cancer xenografts in nude mice. [J]. Journal of Chongqing Medical University 36(5):546-549(2011) DOI： 10.13406/j.cnki.cyxb.2011.05.018.

摘要

目的:探讨重组人血管内皮抑素（Recombinanthumanendostatin

rh-ES）联合长春瑞滨+顺铂（NP方案）化疗对人乳腺癌裸鼠移植瘤的肿瘤生长及新生血管生成的抑制作用。方法:将40只荷瘤裸鼠随机分成NP方案组（A组）、rh-ES组（B组）、联合用药组（C组）和空白对照组（D组）

每组10只。A组给予NP方案化疗1周期

B组给予rh-ES 2周期

C组给予rh-ES两周期加NP方案1周期

D组给予等剂量生理盐水。其中

rh-ES连续用药14 d为1周期

间隔7 d后重复1周期。计算各组抑瘤率

绘制肿瘤生长曲线。肿瘤组织进行免疫组化法染色

测各组血管内皮生长因子（Vascular endothelial growth factor

VEGF）及微血管密度（Microvascular density

MVD）的表达。结果:A、B、C 3组抑瘤率分别为61.80%、64.79%、88.95%

C组抑瘤率明显高于其它两组。C组与A、D两组比较VEGF表达

具有显著的统计学差异（P值均为0.001）。C组与A、B、D 3组比较MVD

P值分别为0.000

0.006和0.000

均具有统计学意义。结论:rh-ES联合NP方案化疗能明显抑制人乳腺癌裸鼠移植瘤生长及新生血管生成

且两者联用具有协同作用。

Abstract

Objective:To evaluate the inhibitory effect of recombinant human endostatin(rh-ES) combined with NP treatment on tumor growth and antiagiogenesis in nude mice after xenograft of MCF-7 cell line.Methods:40 nude mice models with human breast carcinoma were divided into group NVB-Cisplatin(group A)

group rh-ES(group B)

group NVB-Cisplatin + rh-ES(group C)and control group(group D)

each group had 10 nude mice.Group A was given NP treatment one cycle.Group B was given rh-Endostatin two cycles.Group C was given NP treatment one cycle combined with rh-Endostatin two cycles.Group D was given normal saline solution respectively.Rh-Endostatin was used two cycles

each cycle for 14 days

two cycles interval 7 days.Tumor inhibition rate(TIR)and growth curve of each group was calculated and plotted.The vascular endothelial growth factor(VEGF)and microvascular density(MVD)expression were detected by immunohistochemistry.Results:TIR of A

B and C groups were 61.80%

64.79% and 88.95%.The TIR of group C was obviously higher than other two groups.Compared with VEGF expression

group C was significantly lower than group A and group D(both P were 0.001).Compared with MVD

group C was significantly lower than other three groups.Conclusion:Rh-Endostatin combined NP treatment have synergistic effect

they can obviously inhibit tumor growth and angiogenesis.

关键词

乳腺癌MCF-7细胞株重组人血管内皮抑素血管内皮生长因子微血管密度

Keywords

breast cancerMCF-7 cell linerecombinant human endostatinVEGFMVD

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Related Institution

Department of Oncology,the First Affiliated Hospital,Chongqing Medical University

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