ZHENG Pei-yan, SUN Bao-qing, HUANG Hui-min, et al. Capture ELISA and FEIA assay for allergen-specific IgE antibody:a comparative analysis. [J]. Journal of Chongqing Medical University 36(5):595-598(2011)
ZHENG Pei-yan, SUN Bao-qing, HUANG Hui-min, et al. Capture ELISA and FEIA assay for allergen-specific IgE antibody:a comparative analysis. [J]. Journal of Chongqing Medical University 36(5):595-598(2011) DOI: 10.13406/j.cnki.cyxb.2011.05.007.
Capture ELISA and FEIA assay for allergen-specific IgE antibody:a comparative analysis
Objective:Enzymes immune to the capture of the law ALLERG-O-LIQ system and enzyme immune to the fluorescence detection method ImmunoCAP system allergic IgE the specifisity antibodies of observation.in contrast.Methods:The object of study is for the patient’s cases 2008-2010 in allergies and pediatrics departments.Adopt two different enzymes immune to the original specificity IgE antibodies detect.There are 9 kinds of allergic
consist of D.pteronyssinus(D1)
Dermatophagus farinae(D2)
Dog dander(E3)
Blattella Germanica(I6)Egg(F1)
Milk(F2)
Peanuts(F13)
Crub(F23)
Shrimp(F24).And the two inspection results in linear regression tests and consistency.Results:There are 520 times allergic to the original specificity IgE detect.Two systems for inhaling of the antibodies specificity IgE an allergy test results of consistency good(P<0.05)
specificity IgE result:D.pteronyssinus > Dermatophagus farinae > Blattella Germanica > Dog dander
but with food allergy test results of the antibodies sige relevance more into allergic to the low
Specificity IgE Result:Crub>Shrimp> Peanuts>Egg>Milk.Conclusion:ALLERG-O-LIQ syaterm and ImmunoCAP systerm both belong to the new generation is allergic to the operation of the detection system
Two detection methods consistent
from a relatively small
a linear regression distribution
So
with the serum Specificity IgE systerm of ALLERG-O-LIQ is a reliable
economic and clinical diagnosis.The test results and ImmunoCAP system compatible test results.