Mechanisms of TMP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8

YIN Juan; et al

doi:10.13406/j.cnki.cyxb.2011.04.028

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Mechanisms of TMP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8

更新时间：2024-01-17

- Mechanisms of TMP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8
- Journal of Chongqing Medical University Vol. 36, Issue 4, Pages: 401-404(2011)
- 作者机构：
  
  1. 重庆医科大学附属第二医院妇产科
  2. 重庆医科大学生命科学研究院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.04.028
  CLC： R737.31
- Published：2011，
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[1]殷娟,于超,杨竹.川芎嗪抑制IL-8诱导人卵巢癌SKOV3细胞的迁移作用[J].重庆医科大学学报,2011,36(04):401-404.

YIN Juan, et al. Mechanisms of TMP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8. [J]. Journal of Chongqing Medical University 36(4):401-404(2011)
[1]殷娟,于超,杨竹.川芎嗪抑制IL-8诱导人卵巢癌SKOV3细胞的迁移作用[J].重庆医科大学学报,2011,36(04):401-404. DOI： 10.13406/j.cnki.cyxb.2011.04.028.

YIN Juan, et al. Mechanisms of TMP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8. [J]. Journal of Chongqing Medical University 36(4):401-404(2011) DOI： 10.13406/j.cnki.cyxb.2011.04.028.

摘要

目的:观察外源白介素-8（Interleukin-8

IL-8）对人卵巢癌SKOV3细胞迁移的影响

及川芎嗪（Tetramethylpyrazine

TMP）对它的干预作用

初步研究其可能机制。方法:TMP处理SKOV3细胞后

MTT法确定药物浓度;细胞划痕实验观察外源IL-8对SKOV3细胞迁移能力的影响

采用Transwell法观察TMP对IL-8诱导SKOV3细胞迁移的干预作用;Western blot分析SKOV3细胞中E-cadherin和基质金属蛋白酶-9（Matrix metalloproteinase-9

MMP-9）的蛋白表达变化。结果:经IL-8（100 ng/ml）处理后

SKOV3细胞迁移力增加。TMP（50、100μg/ml）具有抑制IL-8诱导的SKOV3细胞迁移作用。IL-8使SKOV3细胞中E-cadherin的表达显著降低（40.2±5.3）%

MMP-9的蛋白表达显著增加（34.6±4.4）%;而TMP可以上调E-cadherin的表达（74.9±6.4）%

同时使MMP-9的蛋白表达显著降低（86.5±5.8）%。结论:川芎嗪可以抑制IL-8诱导的SKOV3细胞迁移作用与细胞中E-cadherin和MMP-9蛋白表达变化有关。

Abstract

Objective:To investigate whether tetramethylpyrazine(TMP)has the activity of inhibition on cell migration induced by extraneous Interleukin-8(IL-8) in SKOV3 cell

and to understand its probable mechanisms.Methods:MTT was used to determine the concentrations of TMP.The wound healing assay and transwell assay were used to study the cell migration.The protein expression levels of E-cadherin and matrix metalloproteinase 9(MMP-9) were detected by Western blot.Results:IL-8(100 ng/ml)increased the ability of cell migration in SKOV3 cell

TMP(50

100 μg/ml)inhibited this process.IL-8 down-regulated the protein expression of E-cadherin(40.2±5.3)%

and up-regulated the protein expression of MMP-9(34.6±4.4)% respectively and significantly

and TMP(50

100 μg/ml)increased the expression of E-cadherin(74.9±6.4)% and decreased the expression of MMP-9(86.5±5.8)% in SKOV3 cells.Conclusion:TMP inhibits cell migration induced by extraneous IL-8

which correlates with variable protein expression changes of E-cadherin and MMP-9 in SKOV3 cells.

关键词

川芎嗪卵巢癌IL-8细胞迁移

Keywords

tetramethylpyrazine(TMP)ovarian carcinomainterleukin-8(IL-8)cell migration

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