Effect of ΔNp63 on invasion in TCCB 5637 cells

JING Peng; et al

doi:10.13406/j.cnki.cyxb.2011.04.027

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Effect of ΔNp63 on invasion in TCCB 5637 cells

更新时间：2024-01-17

- Effect of ΔNp63 on invasion in TCCB 5637 cells
- Journal of Chongqing Medical University Vol. 36, Issue 4, Pages: 426-429(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学基础医学院检验系
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.04.027
  CLC： R737.14
- Published：2011，
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[1]敬鹏,吴小候,罗春丽,陈刚,何云峰.ΔNp63基因对TCCB 5637细胞侵袭作用的影响[J].重庆医科大学学报,2011,36(04):426-429.

JING Peng, et al. Effect of ΔNp63 on invasion in TCCB 5637 cells. [J]. Journal of Chongqing Medical University 36(4):426-429(2011)
[1]敬鹏,吴小候,罗春丽,陈刚,何云峰.ΔNp63基因对TCCB 5637细胞侵袭作用的影响[J].重庆医科大学学报,2011,36(04):426-429. DOI： 10.13406/j.cnki.cyxb.2011.04.027.

JING Peng, et al. Effect of ΔNp63 on invasion in TCCB 5637 cells. [J]. Journal of Chongqing Medical University 36(4):426-429(2011) DOI： 10.13406/j.cnki.cyxb.2011.04.027.

摘要

目的:研究ΔNp63基因对TCCB5637细胞侵袭功能的影响。方法:针对ΔNp63基因的不同靶点设计并化学合成具有阳性转染率的siRNA

用脂质体法转染TCCB 5637细胞

并根据转染的方式将实验分组为:空白对照组、空载体组、阴性质粒组和干扰质粒组;采用实时荧光定量PCR、Western blot检测各组中ΔNp63的mRNA、蛋白的表达;采用激光共聚焦显微镜对转染干扰质粒前后的ΔNp63蛋白进行定位;采用细胞计数法及MTT法来检测ΔNp63基因对TCCB 5637细胞侵袭能力和异质粘附能力的影响。结果:ΔNp63蛋白定位于TCCB 5637细胞的细胞核区域;与空白对照组比较

空载体组和阴性质粒组TCCB5637细胞中ΔNp63的mRNA、蛋白的表达水平无明显变化（P>0.05）;平均细胞穿越数和异质粘附性无明显变化（P>0.05）;而干扰质粒组TCCB5637细胞中ΔNp63的mRNA、蛋白的表达水平明显降低（P<0.05）

平均细胞穿越数明显增加（P<0.05）

异质粘附性明显下降（P<0.05）。结论:ΔNp63基因能明显促进TCCB5637细胞的侵袭能力。

Abstract

Objective:To explore the effects of ΔNp63 on invasion in TCCB 5637 cells in vitro.Methods:siRNA were designed according to different targets on ΔNp63 gene were transfected into TCCB 5637 cells by lipidosome method.The TCCB 5637 cells were divided into control group(without transfection)

vector group(without transfection but added lipo2000)

negative plasmid group(with transfection of siRNA negative)and the interference plasmid(with transfection of effective siRNA).The stable colonies were obtained by G418 screening.Laser confocal microscopy was used to detect the location ofΔNp63 in the 5637 cells;The expression level of ΔNp63 was assayed by real-time PCR and Western blot.The invasion of cell was detected by Transwell.The change of cell adhesion was analyzed by cell adhesion assay.Results:The ΔNp63 proteins were localized on the perinuclear region;The expression of ΔNp63 was obviously decreased in the interference plasmid group;in which cell adhesion was decrease as well as the invasion increased significantly.Statistics analysis shows the interference plasmid group has significant difference compared with other groups.Conclusion:The ΔNp63 gene can significantly increased the function of invasion in TCCB 5637 cells.

关键词

肿瘤ΔNp63siRNA侵袭

Keywords

tumorΔNp63siRNAinvasion

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