Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose

SHEN Zhao-nan; et al

doi:10.13406/j.cnki.cyxb.2011.04.026

您当前的位置：

首页 >

文章列表页 >

Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose

更新时间：2024-01-17

- Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose
- Journal of Chongqing Medical University Vol. 36, Issue 4, Pages: 412-415(2011)
- 作者机构：
  
  重庆医科大学附属第一医院肾内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.04.026
  CLC： R692.6
- Published：2011，
扫描看全文
[1]沈赵楠,甘华,杜晓刚,王晓林.PFT-α对高糖诱导的肾小球足细胞泛素化通路和细胞凋亡的影响[J].重庆医科大学学报,2011,36(04):412-415.

SHEN Zhao-nan, et al. Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose. [J]. Journal of Chongqing Medical University 36(4):412-415(2011)
[1]沈赵楠,甘华,杜晓刚,王晓林.PFT-α对高糖诱导的肾小球足细胞泛素化通路和细胞凋亡的影响[J].重庆医科大学学报,2011,36(04):412-415. DOI： 10.13406/j.cnki.cyxb.2011.04.026.

SHEN Zhao-nan, et al. Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose. [J]. Journal of Chongqing Medical University 36(4):412-415(2011) DOI： 10.13406/j.cnki.cyxb.2011.04.026.

摘要

目的:观察p53抑制剂（Pifithrin-alpha

PFT-α）对高糖刺激后肾小球足细胞泛素化通路和细胞凋亡的影响。方法:体外培养小鼠肾小球足细胞

分为对照组、高糖组（30 mmol/L葡萄糖）、甘露醇组（30 mmol/L葡萄糖+25 mmol/L甘露醇）、PFT-α组（30 mmol/L葡萄糖+10μmol/LPFT-α）、0.2%二甲基亚砜（Dimethyl sulfoxide

DMSO）溶剂对照组（30 mmol/L葡萄糖+0.2%DMSO）。用Western blot法检测各组足细胞p53和COP1蛋白的表达

TUNEL方法检测各组足细胞凋亡情况。结果:高糖刺激48 h后足细胞p53蛋白表达水平（1.210 8±0.035）较对照组（0.072 9±0.007 13）明显增加

COP1蛋白表达水平（0.579 6±0.041 9）则较对照组（1.067±0.053 4）下调;PFT-α在高糖刺激48 h后作用足细胞6 hp

53蛋白表达水平（0.166 4±0.038 7）较高糖组（1.210 8±0.035）下调

COP1蛋白表达水平（0.308 1±0.014 5）与高糖组（0.579 6±0.041 9）则差异无统计学意义;高糖刺激足细胞后凋亡率（35.41±3.31）%分别是对照组（2.23±0.17）%和PFT-α组（4.27±0.21）%的15.87倍和8.29倍

甘露醇组（42.44±6.11）%和0.2%DMSO组（32.80±5.02）%与高糖组差异无统计学意义。结论:高糖损伤肾小球足细胞时可能抑制小鼠肾小球足细胞泛素蛋白酶体活化

进而增加肾小球足细胞的凋亡

PFT-α可通过下调p53蛋白致小鼠肾小球足细胞凋亡减少。

Abstract

Objective:To investigate the effect of pifithrin-α(PFT-α)on the ubiquitin and the apoptosis of podocytes by high glucose.Methods:Cultured mouse podocytes were divided into normal group

high glucose group(30 mmol/L D-glucose)

mannital group(30 mmol/L D-glucose+25 mmol/L mannital)

PFT-α group(30 mmol/L D-glucose+10 μmol/L PFT-α)and 0.2% DMSO group(30 mmol/L glucose+0.2% DMSO).The expressions of p53 and COP1 protein of each group were measured by Western blot.The apoptosis of podocytes was measured by TUNEL.Results:The expression of p53 in mouse podocytes by high glucose in 48 hours(1.2108±0.035)was higher than that in the normal group(0.072 9±0.007 13)

but the expression of COP1(0.579 6±0.041 9)was lower than that of normal group(1.067±0.053 4).After PFT-α affected podocytes for 6 hours after high glucose

the expression of p53 in mouse podocytes(0.166 4±0.038 7)was lower than that in the high glucose group(1.210 8±0.035)

the expression of COP1(0.308 1±0.014 5)was the same as high glucose group(0.579 6±0.041 9).The apoptosis of podocytes in high glucose group was(35.41±3.31)%

which was 15.87 times higher than that of normal group(2.23±0.17)% and 8.29 times higher than that of PFT-α group(4.27±0.21)% respevtively

mannital group(42.44±6.11)% and 0.2% DMSO group(32.80±5.02)% were the same as high glucose group.Conclusion:High glucose injured podocytes may inhibit the activity of ubiquitin proteasome and increase the apoptosis of mouse podocytes.PFT-α can decrease the apoptosis through decreasing the expression of p53.

关键词

肾小球足细胞高糖p53COP1泛素化

Keywords

podocytehigh glucosep53COP1ubiquitin

references

Views

205

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

IL-17-JAK2/STAT3 signaling pathway reduces chemo-induced apoptosis in hepatocellular carcinoma through regulating p53

Effects of HIF1α-KIM1 signaling pathway on renal interstitial fibrosis in diabetic nephropathy

子宫内膜良恶性病变bcl－2，p53蛋白表达的免疫组化研究

The expression and significance of p53, C-erbB-2 and ER in endometrial cancer

P53 overexpression and mutation in endometrial carcinoma

Related Author

Wu Jinghua

Li Leilei

Guo Jiapei

Guo Bin

Dang Yong

Li Yaqian

Yu Fang

彭真年

Related Institution

Department of Laboratory,the Affiliated Hospital of North China University of Science and Technology

The First Clinical Medical College of Nanchang University

Department of Nephrology,Anyang People’s Hospital Henananyang

第二临床学院病理科

北京医科大学人民医院妇产科

⁰