Association of single nucleotide polymorphisms and combination genotype distributions of the KL gene with essential hypertension in the aged

LIU Xiao-lin; et al

doi:10.13406/j.cnki.cyxb.2011.04.013

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Association of single nucleotide polymorphisms and combination genotype distributions of the KL gene with essential hypertension in the aged

更新时间：2024-01-17

- Association of single nucleotide polymorphisms and combination genotype distributions of the KL gene with essential hypertension in the aged
- Journal of Chongqing Medical University Vol. 36, Issue 4, Pages: 389-393(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院老年病科
  2. 重庆医科大学附属第一医院耳鼻喉科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.04.013
  CLC： R544.1
- Published：2011，
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[1]刘晓林,马厚勋,田小春,吴平,周平,吴蔚.KL基因单核苷酸多态性及其组合与老年高血压的相关性研究[J].重庆医科大学学报,2011,36(04):389-393.

LIU Xiao-lin, et al. Association of single nucleotide polymorphisms and combination genotype distributions of the KL gene with essential hypertension in the aged. [J]. Journal of Chongqing Medical University 36(4):389-393(2011)
[1]刘晓林,马厚勋,田小春,吴平,周平,吴蔚.KL基因单核苷酸多态性及其组合与老年高血压的相关性研究[J].重庆医科大学学报,2011,36(04):389-393. DOI： 10.13406/j.cnki.cyxb.2011.04.013.

LIU Xiao-lin, et al. Association of single nucleotide polymorphisms and combination genotype distributions of the KL gene with essential hypertension in the aged. [J]. Journal of Chongqing Medical University 36(4):389-393(2011) DOI： 10.13406/j.cnki.cyxb.2011.04.013.

摘要

目的:研究重庆地区老年汉族人群克老素（Klotho

KL）基因3个单核苷酸多态性及其组合分布规律

揭示其与老年高血压的相关性。方法:同期选择老年原发性高血压病人775例

正常健康老年人339例（年龄≥60岁）作为研究对象。提取外周血白细胞基因组DNA

应用单一等位基因特异性引物PCR技术（SASP-PCR）检测KL基因3个SNP位点的基因型

分析各等位基因及其组合与高血压病的相关性。结果:高血压病组KL基因G-395ASNP AA等位基因频率较对照组增高（P<0.01）

若按性别分层研究

高血压组男性患者其AA等位基因型频率较对照组明显增高（P=0.001）。高血压病组KL基因C370S SNP及F352V SNP各等位基因型频率较对照组均无显著性差异（P>0.05）。组合分布研究发现:高血压病组KL G-395A+F352V+C370C、KL A-395A+F352V+C370C与KL A-395A+F352V+C370S组合基因型频率较对照组明显增高（P<0.05）

按性别分层研究则发现仅在高血压病组男性患者其KL G-395A+F352V+C370C、KL A-395A+F352V+C370C及KL A-395A+F352V+C370S组合分布基因型频率较对照组增高有显著性差异（P<0.05）。本研究还发现在高血压病组男性患者其KLA-395A+F352F+S370S及KLG-395G+V352V+C370S组合分布基因型频率较对照组减低

且有显著性差异（P<0.040）。结论:本文研究发现在老年男性KL基因G-395A SNP与高血压病有明确相关性。组合分布研究还发现KLG-395A+F352V+C370C、KLA-395A+F352V+C370C及KL A-395A+F352V+C370S组合基因型与老年高血压病有关

并存在性别差异性

也就提示携带上述组合基因型的老年男性更易患高血压病。

Abstract

Objective:To investigate the distribution of three cites of single nucleotide polymorphisms(SNPs)and their combination distributions of the KL gene in the Chinese elderly population and analyze their association with hypertension in the aged.Methods:Selected 775 patients with essential hypertension(EH)and 339 heathy people(the age≥60)as research objects.Genomic DNA of leukocytes was extracted from venous blood and the single allele specific primer PCR technique(SASP-PCR)was used to detect allelic genotypes of three sites of SNPs and their distribution of combination genotypes of the KL gene in order to analysis their relations with the EH.Results:The frequency of allele AA genotype of KL G-395A in EH group was higher than that in the control group(P<0.01).According to sex stratification analysis

the frequency of allele AA in the EH male subgroup was obviously higher than that in the control subgroup(P = 0.001).There were no significant difference to be found either in the KL C370S SNP or F352V SNP between EH group and the control group(P>0.5).According to the combination genotype analysis

we found that the allelic genotype frequency of Klotho G-395A+F352V+ C370C

Klotho A-395A+F352V+C370C and Klotho A-395A+F352V+C370S in the EH group was higher than that in the control group(P<0.05)

but according to sex stratification analysis

we only found that the allele genotype frequency of KL G-395A+F352V+ C370C

KL A-395A+F352V+C370C and KL A-395A+F352V+C370S were obviously higher in the EH male subgroup than that in the healthy male subgroup(P<0.05).We also found that the combined allelic genotype frequency of KL A-395A+F352F+S370S and KL G-395G+V352V+C370S were obviously lower in the EH male subgroup than that in the control male subgroup(P<0.04).Conclusion:This study showed that there was an association between SNP of KL G-395A and essential hypertension in the aged.According to the combination genotype analysis

this study found that genotypes of KL G-395A+F352V+C370C

KL A-395A+F352V+C370C and KL A-395A+ F352V+C370S associated with EH in the aged andx difference existed

i.e.the male patients who carried with genotypes of KL G-395A+F352V+C370C

KL A-395A+F352V+ C370C and KL A-395A+ F352V+C370S have a high risk in suffering from EH in the aged.

关键词

Klotho高血压病老年组合基因型

Keywords

Klothoessential hypertensionthe agedcombined genotype

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