Differentiation of PC12 cells induced by total saponin of panax ginseng

LI Han-yu; et al

doi:10.13406/j.cnki.cyxb.2011.04.006

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Differentiation of PC12 cells induced by total saponin of panax ginseng

更新时间：2024-01-17

- Differentiation of PC12 cells induced by total saponin of panax ginseng
- Journal of Chongqing Medical University Vol. 36, Issue 4, Pages: 423-425(2011)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理学教研室
  2. 重庆医科大学干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.04.006
  CLC： R285.5
- Published：2011，
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[1]李晗宇,王顺和,郑娟,姜蓉.人参总皂苷对PC12细胞分化的影响[J].重庆医科大学学报,2011,36(04):423-425.

LI Han-yu, et al. Differentiation of PC12 cells induced by total saponin of panax ginseng. [J]. Journal of Chongqing Medical University 36(4):423-425(2011)
[1]李晗宇,王顺和,郑娟,姜蓉.人参总皂苷对PC12细胞分化的影响[J].重庆医科大学学报,2011,36(04):423-425. DOI： 10.13406/j.cnki.cyxb.2011.04.006.

LI Han-yu, et al. Differentiation of PC12 cells induced by total saponin of panax ginseng. [J]. Journal of Chongqing Medical University 36(4):423-425(2011) DOI： 10.13406/j.cnki.cyxb.2011.04.006.

摘要

目的:研究人参总皂苷（Totalsaponin of panax ginseng

TSPG）诱导PC12细胞神经元性分化的作用。方法:体外培养PC12细胞

观察PC12细胞在TSPG的影响下细胞发生的形态学改变。诱导48 h透射电镜观察突触连接形成情况

72 h利用免疫细胞化学技术

观察TSPG作用后PC12细胞向MAP-2（Microtubule-associated protein 2）阳性细胞分化的情况。结果:TSPG作用后的PC12细胞其突起长度和细胞直径较对照组明显增长和增大[长度由（13.95±2.59）μm增长至（30.33±3.82）μm;直径由（8.25±1.82）μm提高到（14.33±2.84）μm

P<0.05]。TSPG能促进PC12细胞形成突触连接。TSPG组表达MAP2的阳性细胞率明显高于对照组（由4.55%提高到12.22%

P<0.05）。结论:TSPG具有诱导PC12细胞神经元性分化的作用。

Abstract

Objective:To study the potential ability of TSPG(Total saponin of panax ginseng)to induce the neuronal differentiation of PC12 cells.Methods:PC12 cells were cultured in vitro

and their morphological changes under the TSPG were observed.The synaptic linkage were observed by transmitting electronic microscope after the PC12 cells were exposed to TSPG for 48 h and the expression of MAP-2 was detected by immunocytochemistry after 72 h.Results:The length of neurite and max diameter of PC12 cells in TSPG group were obviously longer and bigger than those in control group((13.95±2.59) μm to(30.33±3.82) μm;(8.25±1.82) μm to(14.33±2.84) μm

P<0.05)TSPG could induce PC12 cells to form the synaptic linkage.compared with the control group

the number of MAP-2 positive cells was significantly increased in TSPG group(4.55%to 12.22%

P<0.05).Conclusion:TSPG can induce the neuronal differentiation of PC12 cells.

关键词

人参总皂苷PC12细胞分化

Keywords

total saponin of panax ginsengPC12 celldifferentiation

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