Anti-aging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose

PENG Bin; et al

doi:10.13406/j.cnki.cyxb.2011.04.005

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Anti-aging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose

更新时间：2024-01-17

- Anti-aging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose
- Journal of Chongqing Medical University Vol. 36, Issue 4, Pages: 419-422(2011)
- 作者机构：
  
  1. 川北医学院组织学与胚胎学教研室
  2. 重庆医科大学干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.04.005
  CLC： R285.5
- Published：2011，
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[1]彭彬,陈茂山,蒲莹,王亚平.人参皂苷Rg1延缓D-半乳糖大鼠脑衰老作用及机制的初步研究[J].重庆医科大学学报,2011,36(04):419-422.

PENG Bin, et al. Anti-aging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose. [J]. Journal of Chongqing Medical University 36(4):419-422(2011)
[1]彭彬,陈茂山,蒲莹,王亚平.人参皂苷Rg1延缓D-半乳糖大鼠脑衰老作用及机制的初步研究[J].重庆医科大学学报,2011,36(04):419-422. DOI： 10.13406/j.cnki.cyxb.2011.04.005.

PENG Bin, et al. Anti-aging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose. [J]. Journal of Chongqing Medical University 36(4):419-422(2011) DOI： 10.13406/j.cnki.cyxb.2011.04.005.

摘要

目的:探讨人参皂苷Rg1延缓D-半乳糖所致大鼠脑衰老后的学习记忆能力、SOD活性、MDA含量及衰老相关基因P16INK4a、P21Waf1 mRNA及蛋白的表达水平。方法:将15只3月龄SD大鼠随机分为对照组、脑衰老组[120 mg/（kg.d）D-半乳糖颈背部皮下注射6周]、Rg1抗脑衰老组[同衰老模型组处理

并于第3周起腹腔注射人参皂苷Rg1 20 mg/（kg.d）共注射4周]。Morris水迷宫评估各组大鼠的空间学习记忆能力;比色法测定脑皮质内的SOD活性及MDA含量;实时荧光定量RT-PCR检测海马组织内P16INK4a、P21Cip1/Waf1 mRNA的表达水平;Western blot检测衰老相关基因P16INK4a、P21Cip1/Waf1蛋白的表达。结果:与脑衰老组相比

Rg1抗脑衰老组大鼠学习记忆能力显著提高

脑组织内SOD活性显著升高

MDA含量显著降低

P16INK4a、P21 Cip1/Waf1mRNA及蛋白的表达水平也显著降低。结论:Rg1具有延缓D-半乳糖大鼠脑衰老的作用。其机制可能与增强脑组织的抗氧化能力

下调脑组织内P16INK4a、P21Waf1 mRNA及蛋白的表达水平有关。

Abstract

Objective:To explore the effects of Ginsenoside Rg1 on the spatial learning and memory capacity

the activities of SOD

the amounts of MDA

the expression of P16INK4a and P21Cip1/Waf1 mRNA and protein in brain aging rats induced by D-galactose.Methods:15 normal adult SD rats were randomly divided into control group

brain aging model group and Rg1 anti-brain aging group.In the brain aging model group and the Rg1 anti-brian aging group

D-galactose(120 mg/(kg·d))was given to rats for 6 weeks by subcutaneous injection.Ginsenoside Rg1(20 mg/(kg·d))was also given to rats by intra-peritoneal injection for 4 weeks after 2 weeks in the Rg1 anti-aging group

and saline was given to the control group by subcutaneous and intra-peritoneal injection or to the aging group by intra-peritoneal injection.Learning and memory abilities were measured by a Morris water maze test 42 days after modeling.The activities of SOD and the amounts of MDA in brain were quantified by chromatometry.The changes in the expressions of P16INK4a and P21Cip1/Waf1 mRNA or protein in brain were determined by real time quantitative RT-PCR and Western blot.Results:Compared with the brain aging group

the spatial learning and memory capacities were significantly enhanced;the SOD activities were significantly increased while the MDA level was significantly reduced and the expressions of the P16INK4a or P21Cip1/Waf1 mRNA and protein in brain were reduced in the Rg1 anti-brian aging group.Conclusion:Improvement of the antioxidation ability

downregulation of the expressions of aging-associated P16INK4a and P21Cip1/Waf1 mRNA and protein in brain may be the underlying anti-aging mechanism of Ginsenoside Rg1.

关键词

大鼠人参皂苷Rg1脑衰老D-半乳糖

Keywords

ratsginsenoside Rg1brain agingD-galactose

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