The difference of the ability to synthesize exopolysaccharides of clinical strains of biofilm streptococcus mutans

HUANG Xiao-jing; et al

doi:10.13406/j.cnki.cyxb.2011.03.036

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The difference of the ability to synthesize exopolysaccharides of clinical strains of biofilm streptococcus mutans

更新时间：2024-01-17

- The difference of the ability to synthesize exopolysaccharides of clinical strains of biofilm streptococcus mutans
- Journal of Chongqing Medical University Vol. 36, Issue 3, Pages: 281-285(2011)
- 作者机构：
  
  1. 福建医科大学附属口腔医院牙体牙髓科
  2. 福建医科大学附属协和医院口腔科
  3. 福建医科大学附属口腔医院牙周科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.03.036
  CLC： R781.1
- Published：2011，
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[1]黄晓晶,江山,蔡志宇,闫福华,钟声.生物膜状态变异链球菌临床株合成胞外多糖能力的研究[J].重庆医科大学学报,2011,36(03):281-285.

HUANG Xiao-jing, et al. The difference of the ability to synthesize exopolysaccharides of clinical strains of biofilm streptococcus mutans. [J]. Journal of Chongqing Medical University 36(3):281-285(2011)
[1]黄晓晶,江山,蔡志宇,闫福华,钟声.生物膜状态变异链球菌临床株合成胞外多糖能力的研究[J].重庆医科大学学报,2011,36(03):281-285. DOI： 10.13406/j.cnki.cyxb.2011.03.036.

HUANG Xiao-jing, et al. The difference of the ability to synthesize exopolysaccharides of clinical strains of biofilm streptococcus mutans. [J]. Journal of Chongqing Medical University 36(3):281-285(2011) DOI： 10.13406/j.cnki.cyxb.2011.03.036.

摘要

目的:了解不同致龋力的变异链球菌临床分离株（593号高致龋力临床株和18号低致龋力临床株）在生物膜状态不同时间段合成胞外多糖的能力差异。方法:①在聚苯乙烯塑料片上分别形成3、12、20 h变异链球菌生物膜标本

用异硫氰酸荧光素标记的伴刀豆球蛋白A对其中的胞外多糖进行染色

激光共聚焦扫描显微镜观察。②采用静置法培养形成粘附生长的细菌

蒽酮法测定3～24 h其合成胞外多糖的量。结果:①胞外多糖与染料结合后发出绿色荧光

各时间段593号临床株绿色荧光的分布较18号临床株更致密和广泛。②3～20 h 593号临床株合成水溶性葡聚糖能力强于18号临床株（P<0.05）;3～16 h 593号临床株合成水不溶性葡聚糖能力强于18号临床株（P<0.05）;其他时间点二者合成胞外多糖的能力无显著性差异（P>0.05）。结论:在生物膜形成过程中两菌株合成胞外多糖量随时间延长而增加

593号临床株在生物膜形成早期3～16 h更强的合成胞外多糖能力（含水溶性和水不溶性葡聚糖）

可能是其具高致龋力的原因之一。

Abstract

Objective: To observe the exopolysaccharides produced by biofilm cells of clinical strains 593 and 18 and the difference between the two strains.Methods:① Streptococcus mutans biofilms formed on polystyrene plastic sheets in 3

12 and 20 hours respectively

exopolysaccharides distribution

after fluorescein isothiocyanate-conjugated concanavalin A staining

were visualized by confocal scanning laser microscopy.② The amounts of exopolysaccharides by adhesive streptococcus mutans in different culture time were determined by the anthrone method.Results: After combining with the fluorescent stain

the exopolysaccharides showed green light.Strain 593 in biofilm possessed stronger ability to synthesize exopolysaccharides than strain 18

especially the amount of water soluble glucans in 3～20 h and water insoluble glucans in 3～16 h(P<0.05).Conclusion: The stronger ability of biofilm strain 593 to synthesize exopolysaccharides in the early biofilm formation stage during the 3～16 h may be the reason for its higher cariogenic ability.

关键词

变异链球菌生物膜激光共聚焦扫描显微镜胞外多糖葡聚糖

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