Effect of RNA interference targeted to Livin on the expressions of apoptotic related proteins Caspase-3 and Caspase-9 in esophageal Eca-109 carcinoma cell

CHEN Li; et al

doi:10.13406/j.cnki.cyxb.2011.03.030

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Effect of RNA interference targeted to Livin on the expressions of apoptotic related proteins Caspase-3 and Caspase-9 in esophageal Eca-109 carcinoma cell

更新时间：2024-01-17

- Effect of RNA interference targeted to Livin on the expressions of apoptotic related proteins Caspase-3 and Caspase-9 in esophageal Eca-109 carcinoma cell
- Journal of Chongqing Medical University Vol. 36, Issue 3, Pages: 298-302(2011)
- 作者机构：
  
  重庆医科大学附属第一医院胸心外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.03.030
  CLC： R735.1
- Published：2011，
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[1]陈力,李凡,任国胜.靶向Livin的RNA干扰对食管癌Eca-109细胞凋亡相关蛋白Caspase-3和Caspase-9表达的影响[J].重庆医科大学学报,2011,36(03):298-302.

CHEN Li, et al. Effect of RNA interference targeted to Livin on the expressions of apoptotic related proteins Caspase-3 and Caspase-9 in esophageal Eca-109 carcinoma cell. [J]. Journal of Chongqing Medical University 36(3):298-302(2011)
[1]陈力,李凡,任国胜.靶向Livin的RNA干扰对食管癌Eca-109细胞凋亡相关蛋白Caspase-3和Caspase-9表达的影响[J].重庆医科大学学报,2011,36(03):298-302. DOI： 10.13406/j.cnki.cyxb.2011.03.030.

CHEN Li, et al. Effect of RNA interference targeted to Livin on the expressions of apoptotic related proteins Caspase-3 and Caspase-9 in esophageal Eca-109 carcinoma cell. [J]. Journal of Chongqing Medical University 36(3):298-302(2011) DOI： 10.13406/j.cnki.cyxb.2011.03.030.

摘要

目的:探讨靶向Livin的RNA干扰对食管癌凋亡相关蛋白Caspase-3、Caspase-9的影响。方法:采用慢病毒载体转染Livin高表达的食管癌Eca-109细胞株

从而有效地进行靶向Livin的RNA干扰。分别采用实时荧光定量PCR和Western blot检测各组细胞中Livin、Caspase-3、Caspase-9 mRNA和蛋白表达情况。结果:与空白对照组和阴性组相比

实验组中Livin的mRNA及其蛋白的表达都明显降低（P<0.01）

而Caspase-3和Caspase-9的mRNA及其蛋白的表达都明显增加（P<0.01）。结论:针对Livin的RNA干扰可有效降低Livin高表达的食管癌Eca-109细胞株中Livin mRNA和蛋白水平。同时

与细胞凋亡相关的信号分子Caspase-3和Caspase-9 mRNA和蛋白水平均明显升高。

Abstract

Objective: To investigate the effect of RNA interference targeted to Livin on the expressions of apoptotic related proteins Caspase-3 and Caspase-9 in esophageal Eca-109 carcinoma cell.Methods: Transfection of lentiviral vector was employed to perform effective RNA interference targeted to Livin.Fluorescent quantitative PCR and Western blot was used to determine the mRNA and protein levels of Livin

Caspase-3 and Caspase-9 in groups.Results: The expression of mRNA and protein level of Livin in experimental group was significantly lower than that in normal control group and negative control group(P<0.01).The expression of mRNA and protein levels of Caspase-3 and Caspase-9 in experimental group were significantly higher than those in normal control group and negative control group(P<0.01)

respectively.Conclusion: RNA interference targeted to Livin could decrease the expression of Livin effectively.Meanwhile

the mRNA and protein of apoptosis related signal molecules such as Caspase-3 and Caspase-9 were enhanced significantly.

关键词

RNA干扰食管癌细胞凋亡livinCaspase

Keywords

RNA interferenceesophageal carcinomaapoptosislivinCaspase

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