Objective: To investigate whether the traditional chinese medicine Yisheng injection can reduce the progress of a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury.Methods: 40 female closed colony SD rats were divided into two groups at random.In the control group

the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ for 3 hours before transplantation

while in the experimental group

the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection 0.5 mg/ml at 4℃ for 3 hours before transplantation.After transplantation

the experimental group recipients were treated with Yisheng injection 8 mg/(kg·d) for 9 days.The specimens of blood were harvested from recipients before transplantation and on 1

3

5

7

9 day after transplantation.The changes of MDA and SOD were observed in two groups.Grafts were excised at 12 weeks after transplantation to assess the graft vasculopathy and interstitial fibrosis using HE and Masson staining for two groups.Results:The MDA and SOD of two groups were similar before transplantation.However

the MDA of the two groups increased and the SOD of two groups decreased

especially the first day after transplantation

but it was more significant in the control group.Then

The MDA and SOD of the two groups recovered to normal gradually

but the experimental group recovered to normal on the 5 day after transplantation

faster than the control group

which recovered to normal on the 9 day after transplantation.By 12 weeks

compare the changes of histology of the grafts of the two groups

grafts vasculopathy and interstitial fibrosis were decreased significantly in experimental group.Conclusion: It demonstrated that Yisheng injection can decrease ischemia/reperfusion injury and reduce the development of rat cardiac chronic graft failure in a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury.