The effect of Tanshinol on RhoA/RockⅠsignaling parthway in hepatic stellate cells

JIANG Zhong-hua; et al

doi:10.13406/j.cnki.cyxb.2011.02.040

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The effect of Tanshinol on RhoA/RockⅠsignaling parthway in hepatic stellate cells

更新时间：2024-01-17

- The effect of Tanshinol on RhoA/RockⅠsignaling parthway in hepatic stellate cells
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 168-171(2011)
- 作者机构：
  
  1. 江苏省盐城市第一人民医院消化内科
  2. 重庆医科大学附属第二医院消化内科
  3. 江苏省盐城市第一人民医院消化内科重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.040
  CLC： R965
- Published：2011，
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[1]姜中华,戴立里,余冰冰,李冬.丹参素对肝星状细胞RhoA/RockⅠ信号通路的影响[J].重庆医科大学学报,2011,36(02):168-171.

JIANG Zhong-hua, et al. The effect of Tanshinol on RhoA/RockⅠsignaling parthway in hepatic stellate cells. [J]. Journal of Chongqing Medical University 36(2):168-171(2011)
[1]姜中华,戴立里,余冰冰,李冬.丹参素对肝星状细胞RhoA/RockⅠ信号通路的影响[J].重庆医科大学学报,2011,36(02):168-171. DOI： 10.13406/j.cnki.cyxb.2011.02.040.

JIANG Zhong-hua, et al. The effect of Tanshinol on RhoA/RockⅠsignaling parthway in hepatic stellate cells. [J]. Journal of Chongqing Medical University 36(2):168-171(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.040.

摘要

目的:观察丹参素对肝星状细胞（Hepatic stellate cells

HSC）RhoA/RockⅠ信号通路的影响。方法:体外分离、培养大鼠肝HSC

以RockⅠ特异性抑制剂Y-27632 30μmol/L、0.062 5～0.25 mmol/L丹参素分别与纤维连接蛋白（Fibronectin

FN）刺激的HSC共同孵育。四甲基偶氮唑盐法测定HSC增殖

酶联免疫吸附试验测定Ⅰ型胶原分泌

Western blot测定RhoA

p-Mlc（Thr18/Ser19）p

-FAK（Tyr397）蛋白表达。结果:FN作用24h能刺激HSC增殖

Ⅰ型胶原分泌

且上调了RhoAp、-Mlc（Thr18/Ser19）蛋白

p-FAK（Tyr397）蛋白的表达;丹参素和RockⅠ特异性抑制剂Y-27632均能明显抑制上述效应。结论:FN刺激活化了HSC的RhoA/RockⅠ信号通路、整合素信号通路;丹参素抑制HSC增殖

胶原分泌的作用与阻断RhoA/RockⅠ信号通路有关;RhoA/RockⅠ信号通路阻断后对整合素信号通路关键酶FAK下调效应为丹参素经RhoA/RockⅠ信号通路抗纤维化的分子机制之一。

Abstract

Objective:To investigate the role of Tanshinol on RhoA/RockⅠsignaling parthway in Hepatic Stellate Cells.Methods:The mice of HSCs were isolated.After stimulation of FN

HCSs were cultured in vitro with Tanshinol(0.062 5～0.25 mmol/L)or Y-27632(30 μmol/L)

a specific RockⅠinibitor.The cell proliferation was assessed by MTT assay;the secretion of collagen Ⅰwere examined by methods of Enzyme Linked Immunosorbent Assay;the protein levels of RhoA

p-Mlc(Thr18/Ser19)

and p-FAK(Tyr397) were analyzed by Western Blot.Results:FN promoted the proliferation of HSC significantly and provoked the secretion of collagenⅠmarkedly

meanwhile the protein levels of RhoA

p-Mlc(Thr18/Ser19)

and p-FAK(Tyr397) also increased.The interventions of Y-27632 and Tanshinol could inhibits the above effects in HSC distinctly.Conclusion:FN activates the RhoA/RockⅠsignal parthway and the intergin signal parthway in HSC;Interventing the RhoA/RockⅠsignal parthway might be involved with the effect of Tanshinol that inhibits the HSC proliferation and decreases the secretion of collagenⅠ.The down regulation of FAK by RockⅠis one of the anti-fibrosis mechanisms of Tanshinol.

关键词

丹参素肝星状细胞黏附斑激酶肌球蛋白轻链

Keywords

tanshinolhepatic stellate cellsmyosin light chainsfocal adhesion kinase

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