The characteristic of human dental pulp cells cultured in vitro

WANG Ping; et al

doi:10.13406/j.cnki.cyxb.2011.02.039

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The characteristic of human dental pulp cells cultured in vitro

更新时间：2024-01-17

- The characteristic of human dental pulp cells cultured in vitro
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 174-176(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院口腔科
  2. 重庆医科大学干细胞与组织工程研究室
  3. 重庆医科大学附属口腔医院牙周黏膜病科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.039
  CLC： R781.05
- Published：2011，
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[1]王萍,王亚平,周玥,杨明聪.人牙髓细胞的体外培养及生物学特性研究[J].重庆医科大学学报,2011,36(02):174-176.

WANG Ping, et al. The characteristic of human dental pulp cells cultured in vitro. [J]. Journal of Chongqing Medical University 36(2):174-176(2011)
[1]王萍,王亚平,周玥,杨明聪.人牙髓细胞的体外培养及生物学特性研究[J].重庆医科大学学报,2011,36(02):174-176. DOI： 10.13406/j.cnki.cyxb.2011.02.039.

WANG Ping, et al. The characteristic of human dental pulp cells cultured in vitro. [J]. Journal of Chongqing Medical University 36(2):174-176(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.039.

摘要

目的:从年轻恒牙的牙髓组织中分离培养牙髓细胞

研究其表型及生物学特性。方法:选取因正畸或阻生拔除的年轻健康双尖牙或第三磨牙

取出牙髓

组织块酶消化法分离培养人牙髓细胞

免疫细胞化学检测第3代人牙髓细胞表面标志

并对体外培养的人牙髓细胞的矿化能力进行研究。结果:体外培养的人牙髓细胞表达Ⅰ型胶原及波形丝蛋白

少数细胞增殖可形成克隆并表达间充质干细胞的表面标志STRO-1。体外连续培养可形成钙化结节

加入矿化诱导液后钙化结节形成时间明显提前

牙髓细胞碱性磷酸酶（Alkaline phosphatase

ALP）活性增高。结论:人牙髓细胞中含有少量干细胞

体外培养的人牙髓细胞可向成牙本质细胞分化并形成钙化结节

体外矿化诱导可促进牙髓细胞向成牙本质细胞分化。

Abstract

Objective:To study the characteristic of human dental pulp cells from permanent teeth of young people.Methods:The healthy teeth were selected from the young people because of orthodontics or obstruction.The dental pulp cells were separated and cultured by tissue block enzyme digestion method.Immunohistochemical studies were performed to characterize the pulp cells

and the capacity of mineralization was researched.Results: Collagen type I and Vimentin were expressed in human pulp cells.Colony and STRO-1 were expressed only in several cells.With continued culture

human dental pulp cells formed cell nodus.Applied with odontogenic induction medium

the time of cell nodus was advanced

and alkaline phosphatase activity was increased(P<0.05).Conclusion: There are only a few of human dental pulp stem cells in dental pulp.The continued culture of pulp cells has odontogenic differentiation.

关键词

牙髓细胞细胞培养矿化碱性磷酸酶

Keywords

human dental pulp cellscell culturemineralizationalkaline phosphatase

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