A new assessment method for animal model of choroidal neovascularization (CNV)

XU Qin; et al

doi:10.13406/j.cnki.cyxb.2011.02.037

您当前的位置：

首页 >

文章列表页 >

A new assessment method for animal model of choroidal neovascularization (CNV)

更新时间：2024-01-17

- A new assessment method for animal model of choroidal neovascularization (CNV)
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 192-194(2011)
- 作者机构：
  
  1. 贵州遵义医学院附院检验科
  2. University of Arkansas
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.037
  CLC： R774.5
- Published：2011，
扫描看全文
[1]许琴,Nalini S.Bora,Puran Bora.脉络膜新生血管CNV动物模型的评估新方法[J].重庆医科大学学报,2011,36(02):192-194.

XU Qin, et al. A new assessment method for animal model of choroidal neovascularization (CNV). [J]. Journal of Chongqing Medical University 36(2):192-194(2011)
[1]许琴,Nalini S.Bora,Puran Bora.脉络膜新生血管CNV动物模型的评估新方法[J].重庆医科大学学报,2011,36(02):192-194. DOI： 10.13406/j.cnki.cyxb.2011.02.037.

XU Qin, et al. A new assessment method for animal model of choroidal neovascularization (CNV). [J]. Journal of Chongqing Medical University 36(2):192-194(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.037.

摘要

目的:建立C57BL/6小鼠脉络膜新生血管（Choroidal neovascularization

CNV）动物模型的评估方法。方法:①应用氪红激光对小鼠实验性视网膜进行光凝

激光的功率、光凝斑直径和曝光时间分别为250 mW、50μm及0.05 s诱导CNV动物模型。②分别于光凝后1、3、5、7、10d

用2×106FITC-dextran荧光素灌注小鼠心脏。③mAb特定抗Elastin抗体标记Bruch膜。④视网膜色素上皮（Retinal pigmentepithelium

RPE）-脉络膜-巩膜复合体铺片。⑤激光共聚焦显微镜对RPE-脉络膜-巩膜复合体进行CNV形态进行观察和测量。结果:光凝后3 d可见新生血管及走向清晰可见

光凝后7 d CNV达到高峰。结论:用抗体标记Bruch膜。FITC-dextran荧光素灌注小鼠心脏

激光共聚焦显微镜对RPE-脉络膜-巩膜复合体进行氪红激光诱导C57BL/6小鼠的CNV模型进行评估

直观

可靠。

Abstract

Objective: To establish a new assessment method for animal model of choroidal neovascularization(CNV).Methods: In this model

CNV was induced by laser photocoagulation in C57BL/6 mice with the krypton red laser.Animals from each group were sacrificed and were perfused through the heart with fluorescein-labeled dextran(FITC-Dextran) on day 1

and 7 post laser.Retinal pigment epithelium-choroid-scleral tissue

after using a mAb specific for elastin staining

was examined to determine the incidence and size of CNV complex by confocal microscopy.Results: Confocal micrograph of the neovascular complex in C57BL/6 mice showed the new vessels(green)

and damaged Bruch’s membrane(BM) and RPE were stained red for elastin.CNV appeared on day 3 post laser

reaching the peak on day 7 post laser.Conclusion: The new assessment method of animal model of choroidal neovasculation(CNV) is feasible

reliable and visualized.

关键词

脉络膜新生血管动物模型氪红激光共聚焦显微镜评估

Keywords

choroidal neovascularizationanimal modelkrypton red laserconfocal microscopyassessment

references

Views

213

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of NSA2 in a mouse model of choroidal neovascularization

A mouse model of polycystic ovary syndrome with abnormal glucose and lipid metabolism induced by dihydrotestosterone combined with high-fat diet

Etablishment and evaluation standards of skin photoaging model in SD rat

Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits

Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury

Related Author

CHEN Xin-ke

PENG Hui

LEI Bo

WANG Juan

FU Peng

Wu Jinlin

Zhang Binghan

Long Jiangchuan

Related Institution

Department Of Ophthalmology,the First Affiliated Hospital,Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology

Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

Department of Endocrinology，The Second Affiliated Hospital of Chongqing Medical University

Department of Dermatology,The First Affiliated Hospital of Chongqing Medical University

Department of Vascular Surgery,The First Affiliated Hospital of Chongqing Medical University

⁰