Investigation into the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901

OUYANG Xiao-bo; et al

doi:10.13406/j.cnki.cyxb.2011.02.032

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Investigation into the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901

更新时间：2024-01-17

- Investigation into the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 159-162(2011)
- 作者机构：
  
  重庆医科大学附属第一医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.032
  CLC： R735.2
- Published：2011，
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[1]欧阳小波,郝亚琴,王立.Hedgehog信号通路对人胃癌SGC-7901细胞侵袭迁移作用及机制探讨[J].重庆医科大学学报,2011,36(02):159-162.

OUYANG Xiao-bo, et al. Investigation into the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901. [J]. Journal of Chongqing Medical University 36(2):159-162(2011)
[1]欧阳小波,郝亚琴,王立.Hedgehog信号通路对人胃癌SGC-7901细胞侵袭迁移作用及机制探讨[J].重庆医科大学学报,2011,36(02):159-162. DOI： 10.13406/j.cnki.cyxb.2011.02.032.

OUYANG Xiao-bo, et al. Investigation into the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901. [J]. Journal of Chongqing Medical University 36(2):159-162(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.032.

摘要

目的:探讨Hedgehog（Hh）信号通路对人胃癌SGC-7901细胞侵袭迁移的作用及其机制。方法:环靶明特异性阻断Hh信号通路后

Transwell小室和划痕实验检测SGC-7901细胞侵袭和迁移的能力;RT-PCR检测SGC-7901细胞中MMP-2和MMP-9 mRNA表达;细胞免疫化学检测SGC-7901细胞中MMP-2和MMP-9蛋白表达。结果:阻断Hh信号通路能显著抑制SGC-7901细胞的侵袭和迁移能力（P<0.05）。MMP-2和MMP-9的mRNA以及蛋白表达亦显著降低（P<0.05）。结论:Hh信号通路可能是通过调节MMP-2和MMP-9的表达促进SGC-7901细胞侵袭、迁移。

Abstract

Objective:To investigate the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901.Methods: After cyclopamine blocked Hh signaling pathway specifically

transwell chamber assay and wound healing assay were used to examine the capacities of invasion and migration of SGC-7901 cells respectively.Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detect the expression of MMP-2 and MMP-9 mRNA in SGC-7901 cells.Immunochemistry was used to detect the expression of MMP-2 and MMP-9 protein in SGC-7901 cells.Results: The capacities of invasion and migration of SGC-7901 cells could be significantly inhibited after blocking Hh signaling pathway(P<0.05).There was lower expression of MMP-2 and MMP-9 mRNA than the untreated group in SGC-7901 cells(P<0.05).The expression of MMP-2 and MMP-9 protein also remarkably decreased(P<0.05).Conclusion: Hedgehog signaling pathway may promote invasion and migration of SGC-7901 cells by regulating the expression of MMP-2 and MMP-9.

关键词

Hedgehog胃癌侵袭迁移MMP-2MMP-9

Keywords

hedgehoggastric cancerinvasionmigrationmatrix metalloproteinase-2matrix metalloproteinase-9

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