Radiosensitization effect of arsenic trioxide on human lung cancer cell line A2

REN Qing-lan; et al

doi:10.13406/j.cnki.cyxb.2011.02.031

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Radiosensitization effect of arsenic trioxide on human lung cancer cell line A2

更新时间：2024-01-17

- Radiosensitization effect of arsenic trioxide on human lung cancer cell line A2
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 156-159(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肿瘤科
  2. 重庆市肿瘤医院放疗科
  3. 重庆医科大学基础医学院放射医学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.031
  CLC： R734.2
- Published：2011，
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[1]任庆兰,陈晓品,吴永忠,刘丽,高枫,李少林.三氧化二砷对人肺癌细胞株A2放射增敏的实验研究[J].重庆医科大学学报,2011,36(02):156-159.

REN Qing-lan, et al. Radiosensitization effect of arsenic trioxide on human lung cancer cell line A2. [J]. Journal of Chongqing Medical University 36(2):156-159(2011)
[1]任庆兰,陈晓品,吴永忠,刘丽,高枫,李少林.三氧化二砷对人肺癌细胞株A2放射增敏的实验研究[J].重庆医科大学学报,2011,36(02):156-159. DOI： 10.13406/j.cnki.cyxb.2011.02.031.

REN Qing-lan, et al. Radiosensitization effect of arsenic trioxide on human lung cancer cell line A2. [J]. Journal of Chongqing Medical University 36(2):156-159(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.031.

摘要

目的:本文探讨三氧化二砷（Arsenictrioxide

As2O3）对人肺癌细胞株A2放射敏感性的影响。方法:设立空白对照组、单纯照射组（直线加速器X射线照射

2 Gy）、As2O3组（1.0μmol/LAs2O3）、As2O3+照射组（1.0μmol/LAs2O3+X射线照射

2 Gy）。用流式细胞仪检测As2O3作用后肿瘤细胞周期的变化

MTT法及平板克隆形成试验测定各实验组人肺癌细胞株A2细胞存活分数和集落形成率

用流式细胞仪观察Bcl-2蛋白的表达。结果:As2O3作用后使A2细胞出现明显G2/M期阻滞。As2O3+照射组细胞存活分数和集落形成率较As2O3组及单纯照射组明显下降

与As2O3组或单纯照射组比较差异具有显著性（P<0.01）。As2O3+照射组细胞Bcl-2蛋白的表达明显下调

与As2O3组或单纯照射组比较差异具有显著性（P<0.01）。结论:As2O3对人肺癌细胞A2具有放射增敏作用

其增敏机制可能与As2O3增加照射前G2/M期细胞比例及照射后肿瘤细胞的凋亡率有关。

Abstract

Objective:To explore the effect of arsenic trioxide on radiosensitivity in human lung cancer cell line A2.Methods: There were four groups in the study:control group(untreated group)

As2O3 group(1.0 μmol/L As2O3)

irradiation group

As2O3+ irradiation group.Cellular response to irradiation was evaluated by the colony forming test and MTT assay;Flow cytometry was used to determined the Bcl-2 protein expression and cell cycle.Results: As2O3 could induced a G2/M cell cycle arrest.As2O3 + irradiation could significantly decreased the proliferation rate(vs As2O3 group or irradiation group

P<0.01).As2O3 significantly decreased the expression of Bcl-2 in irradiated human lung cancer cell line A2(vs As2O3 group or irradiation group

P<0.01).Conclusion: Arsenic trioxide could sensitize the human lung cancer cell A2 to ionizing irradiation.This effect is probably caused by increasing the rate of G2/M cells before irradiation and the apoptosis rate of A2 cells after irradiation.

关键词

三氧化二砷人肺癌细胞株A2放射敏感性

Keywords

arsenic trioxidehuman lung cancer cell line A2radiosensitivity

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