Proteomic analysis of serum from patients with polycystic ovary syndrome and insulin resistance

ZHANG Li-jing; et al

doi:10.13406/j.cnki.cyxb.2011.02.023

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Proteomic analysis of serum from patients with polycystic ovary syndrome and insulin resistance

更新时间：2024-01-17

- Proteomic analysis of serum from patients with polycystic ovary syndrome and insulin resistance
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 145-147(2011)
- 作者机构：
  
  重庆医科大学附属第一医院辅助生殖中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.023
  CLC： R711.71
- Published：2011，
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[1]张丽静,丘彦.多囊卵巢综合征胰岛素抵抗差异蛋白的分析[J].重庆医科大学学报,2011,36(02):145-147.

ZHANG Li-jing, et al. Proteomic analysis of serum from patients with polycystic ovary syndrome and insulin resistance. [J]. Journal of Chongqing Medical University 36(2):145-147(2011)
[1]张丽静,丘彦.多囊卵巢综合征胰岛素抵抗差异蛋白的分析[J].重庆医科大学学报,2011,36(02):145-147. DOI： 10.13406/j.cnki.cyxb.2011.02.023.

ZHANG Li-jing, et al. Proteomic analysis of serum from patients with polycystic ovary syndrome and insulin resistance. [J]. Journal of Chongqing Medical University 36(2):145-147(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.023.

摘要

目的:筛选和鉴定与多囊卵巢综合征（Polycysticovarysyndrome

PCOS）胰岛素抵抗（InsulinresistanceI

R）相关的蛋白。方法:采用固相pH梯度双向凝胶电泳分离血清蛋白质

银染显色

通过凝胶成像仪获取图像

PDQuest软件分析电泳图谱

寻找有意义的差异蛋白点

运用MALDI-TOF-MS质谱鉴定

分析其意义。结果:选取具有明显差异的5个蛋白点进行质谱鉴定

经数据库查询

2个蛋白点获得了有意义的结果

分别为结合珠蛋白（Haptoglo-bin

HP）

RhoA蛋白。结论:PCOSIR患者与非胰岛素抵抗（Non-Insulin resistance

non-IR）PCOS妇女血清蛋白表达存在差异

这些差异蛋白有可能是PCOS IR特异相关性蛋白

并有可能成为PCOSIR诊断的分子标志物。

Abstract

Objective: To find out disease specific-proteins(DSPs) which are closely related with polycystic ovary syndrome(PCOS) and insulin resistance(IR).Methods: The serum proteins were separated by immobilized pH gradient-based two-dimensional gel eletrophoresis(2-DE).The gels were stained by silver

scanned by gel formatter

and analyzed by using PDquest software.The differentially displayed protein spots associated with PCOS IR were searched for and identified by MALDI-T0F-MS.Results: Five DSPs were chosen for test and two DSPs were found

which were haptoglobin and RhoA protein.Conclusion:There are different protein expressions in 2D-PAGE of PCOS IR and non-IR PCOS.These proteins may be DSPs of PCOS IR

and may be the markers for diagnosis.

关键词

多囊卵巢综合征胰岛素抵抗血清蛋白

Keywords

polycystic ovary syndromeinsulin resistanceserumprotein

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Related Institution

Department of Endocrinology,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第一医院内分泌科

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重庆市第三人民医院内分泌科

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