Effects of putrescine on Atp8a1 gene expression in mouse NTH3T3 cells

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Effects of putrescine on Atp8a1 gene expression in mouse NTH3T3 cells

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- Effects of putrescine on Atp8a1 gene expression in mouse NTH3T3 cells
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 133-135(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院呼吸科
  2. 重庆医科大学感染性疾病分子生物学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.010
  CLC： R341
- Published：2011，
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[1]万涛,李朝幸,田园园,王李英,秦栋栋,李凯,曲嘉琳,汤华.腐胺对小鼠成纤维细胞中Atp8a1基因表达的影响[J].重庆医科大学学报,2011,36(02):133-135.

WAN Tao, et al. Effects of putrescine on Atp8a1 gene expression in mouse NTH3T3 cells. [J]. Journal of Chongqing Medical University 36(2):133-135(2011)
[1]万涛,李朝幸,田园园,王李英,秦栋栋,李凯,曲嘉琳,汤华.腐胺对小鼠成纤维细胞中Atp8a1基因表达的影响[J].重庆医科大学学报,2011,36(02):133-135. DOI： 10.13406/j.cnki.cyxb.2011.02.010.

WAN Tao, et al. Effects of putrescine on Atp8a1 gene expression in mouse NTH3T3 cells. [J]. Journal of Chongqing Medical University 36(2):133-135(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.010.

摘要

目的:研究腐胺对小鼠成纤维细胞中Atp8a1基因表达的影响

初步探讨其作用机制。方法:用基因芯片和Real-timePCR检测正常小鼠和Azin1（Antizyme inhibitor 1）基因敲除小鼠肝脏组织中Atp8a1基因的在mRNA水平上的表达;构建Atp8a1基因启动子的虫荧光素酶报告质粒pGL3-Atp8a1-P;将重组质粒转染NTH3T3成纤维细胞中

分别在含有4μg/ml腐胺和不含腐胺的条件下

用双荧光素酶检测系统检测虫荧光素酶活性。结果:在含有腐胺的培养条件下

转染Atp8a1基因启动子虫荧光素酶报告质粒的细胞虫荧光素酶的活性明显改变。结论:腐胺能够抑制Atp8a1基因启动子活性而降低其在NIH3T3成纤维细胞中的表达。

Abstract

Objective: To investigate the effects of putrescine on the expression of Atp8a1 in NIH3T3 cells and reveal its regulatory mechanism.Methods: DNA microarray and Real-time PCR were performed to detect the Atp8a1 gene expression in normal mouse liver and Azin1 knockout mouse liver.Atp8a1 promoter luciferase reporter plasmid

pGL3-Atp8a1-P

was constructed.NTH3T3 cells were transiently transfected with pGL3-Atp8a1-P

and cultured in medium with and without putrescine respectively.Then the luciferase activity was detected.Results: The relative luciferase activity was changed in the cells which were transfected with pGL3-Atp8a1-P when cultured in medium added with putrescine.Conclusion: Putrescine could down-regulate the Atp8a1 gene expression in NIH3T3 cells by inhibiting its promoter’s activity.

关键词

腐胺Atp8a1启动子基因表达Azin1基因敲除小鼠

Keywords

putrescineAtp8a1promotergene expressionAzin1 knockout mouse

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