The characterization of norovirus genotypes in children in Chongqing

LIAO Yang; et al

doi:10.13406/j.cnki.cyxb.2011.02.001

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The characterization of norovirus genotypes in children in Chongqing

更新时间：2024-01-17

- The characterization of norovirus genotypes in children in Chongqing
- Journal of Chongqing Medical University Vol. 36, Issue 2, Pages: 242-245(2011)
- 作者机构：
  
  重庆医科大学附属儿童医院感染消化科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.02.001
  CLC： R373
- Published：2011，
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[1]廖炀,陈军华,刘作义,彭哲,幸琳琳,张爱华,朱朝敏.重庆地区儿童诺如病毒基因型别特点研究[J].重庆医科大学学报,2011,36(02):242-245.

LIAO Yang, et al. The characterization of norovirus genotypes in children in Chongqing. [J]. Journal of Chongqing Medical University 36(2):242-245(2011)
[1]廖炀,陈军华,刘作义,彭哲,幸琳琳,张爱华,朱朝敏.重庆地区儿童诺如病毒基因型别特点研究[J].重庆医科大学学报,2011,36(02):242-245. DOI： 10.13406/j.cnki.cyxb.2011.02.001.

LIAO Yang, et al. The characterization of norovirus genotypes in children in Chongqing. [J]. Journal of Chongqing Medical University 36(2):242-245(2011) DOI： 10.13406/j.cnki.cyxb.2011.02.001.

摘要

目的:了解重庆地区儿童诺如病毒（Norovirus

NV）基因型别特点。方法:收集2008年8月至2009年7月全年轮状病毒抗原阴性的急性非细菌性腹泻标本

用real-time RT-PCR方法扩增NV ORF1-ORF2结合区

测序扩增片段后进行比对并分型及构建系统进化树。结果:全年获得806份标本

共检测出251份为NV阳性标本

其中GⅠ组4例

GⅡ组247例。NV以7

0月份为高发月份

以6～12月龄婴儿最多见。已分型的NV GⅡ基因组（108例）以GⅡ.4/2006b占绝对优势

其构成比达62.96%（68/108）

其余次为GⅡ.3占25.93%（28/108）;GⅡ.6占6.48%（7/108）;GⅡ.2占1.85%（2/108）;此外还分别检测出GⅡ.7、GⅡ.12、GⅡ.13各有1例（1/108）。结论:重庆地区NV夏季发病率较高

流行毒株以GⅡ.2006b为主。

Abstract

Objective: To investigate the characteristics of norovirus genotype of children in Chongqing.Methods: Stool specimens of nonbacterial acute diarrhea with rotavirus antigen-negative were collected from August 2008 to July 2009.Real-time RT-PCR was used to detect the ORF1-ORF2 junction regions of norovirus.The sequencing fragments of RT-PCR products were blasted in GenBank and classified.Phylogenetic trees were constructed by the neighbor-joining method.Results: 251 of 806 stool specimens were detected for the norovirus-positive

of which 4 cases belonged to GⅠ group and 247

GⅡ group.It was most likely to occur in summer(from July to October)and in 6~12 months children.Among the sub-genotypes of GⅡ group(108 cases had been genotyped successfully)

GⅡ.4/2006b was dominant

with a constituent ratio of 62.96%(68/108)

and GⅡ.3 accounted for 25.93%(28/108);GⅡ.6

6.48%(7/108);GⅡ.2

1.85%(2/108).Besides

GⅡ.7

GⅡ.12

and GⅡ.13 were also detected.Conclusion: The prevalence of norovirus is found higher in summer in Chongqing

and GⅡ.2006b is the dominant genotype.

关键词

诺如病毒基因分型婴幼儿分子流行病学RT-PCR

Keywords

norovirusgenotypeinfantmolecular epidemiologyRT-PCR

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