Histone deacetylase inhibitors down-regulate hepatic expression of adhesion molecules in septic mice

WANG Bin; et al

doi:10.13406/j.cnki.cyxb.2011.01.037

您当前的位置：

首页 >

文章列表页 >

Histone deacetylase inhibitors down-regulate hepatic expression of adhesion molecules in septic mice

更新时间：2024-01-17

- Histone deacetylase inhibitors down-regulate hepatic expression of adhesion molecules in septic mice
- Journal of Chongqing Medical University Vol. 36, Issue 1, Pages: 8-11(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院麻醉科
  2. 重庆医科大学基础医学院病理生理学教研室
  3. 重庆医科大学药学院药理学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.01.037
  CLC： R459.7
- Published：2011，
扫描看全文
[1]王彬,张力,万敬员,姜蓉,闵苏.组蛋白去乙酰酶抑制剂下调脓毒症小鼠肝内粘附分子表达[J].重庆医科大学学报,2011,36(01):8-11.

WANG Bin, et al. Histone deacetylase inhibitors down-regulate hepatic expression of adhesion molecules in septic mice. [J]. Journal of Chongqing Medical University 36(1):8-11(2011)
[1]王彬,张力,万敬员,姜蓉,闵苏.组蛋白去乙酰酶抑制剂下调脓毒症小鼠肝内粘附分子表达[J].重庆医科大学学报,2011,36(01):8-11. DOI： 10.13406/j.cnki.cyxb.2011.01.037.

WANG Bin, et al. Histone deacetylase inhibitors down-regulate hepatic expression of adhesion molecules in septic mice. [J]. Journal of Chongqing Medical University 36(1):8-11(2011) DOI： 10.13406/j.cnki.cyxb.2011.01.037.

摘要

目的:研究组蛋白去乙酰化酶抑制剂对脓毒症小鼠肝内粘附分子表达及炎症细胞浸润的影响。方法:小鼠经腹腔注射组蛋白去乙酰化酶抑制剂（Trichostatin A或丁酸钠）后

行盲肠结扎穿孔术以诱导小鼠脓毒症。18 h后处死小鼠并收集血清和肝组织

检测血清转氨酶活性及肝组织匀浆髓过氧化物酶活性;常规石蜡切片HE染色观察组织病理学改变;免疫组织化学法检测肝组织内粘附分子（细胞间粘附分子-1及E-选择素）的表达水平。结果:2种结构不相关的组蛋白去乙酰化酶抑制剂均可下调脓毒症小鼠肝组织内粘附分子的表达

而肝组织HE染色切片上也观察到组蛋白去乙酰化酶抑制剂处理组肝内炎症细胞浸润明显减轻

与此一致

组蛋白去乙酰化酶抑制剂处理组肝组织匀浆中髓过氧化物酶活性及血清转氨酶活性显著降低。结论:组蛋白去乙酰化酶抑制剂处理组可下调脓毒症小鼠肝内粘附分子表达

从而减轻炎症细胞浸润和肝组织损伤。

Abstract

Objective:To investigate the effects of histone deacetylase(HDAC) inhibitors on the expression of adhesion molecules in liver tissue and the infiltration of leukocytes in liver tissue during the progression of sepsis.Methods:C57BL/6 mice were pre-treated with two structure unrelated HDAC inhibitors

Trichostatin A or sodium butyrate

and then sepsis was induced by cecal ligation and puncture(CLP).At 18 h after CLP

hepatic expression of adhesion molecules

including intercellular adhesion molecule-1(ICAM-1) and E-selectin

were detected by immunohistochemical analysis.The formalin-fixed liver specimens were also stained with HE routinely for morphologic evaluation.In addition

myeloperoxidase(MPO) activity in liver tissue and serum aminotransferases levels were measured.Results:Treatment with HDC inhibitors down-regulated the expression of adhesion molecules.This was accompanied by reduced leukocyte infilatraion in the liver tissue

decreased MPO activity in hepatic homogenates as well as suppressed elevation of serum aminotransferases levels.Conclusion:HDAC inhibitors effectively suppressed the expression of adhesion molecules

thus significantly attenuated the recruitment of leukocytes and alleviated hepatic injury induced by sepsis.

关键词

组蛋白去乙酰化酶脓毒症粘附分子炎症盲肠结扎穿孔术

Keywords

histone deacetylasesepsisadhesion moleculeinflammationcecum ligation and puncture

references

Views

132

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Endothelial-overexpressed lipopolysaccharide-associated factor 1 promotes skin wound healing in diabetic mice by affecting the nuclear factor-kappa B signaling pathway

Protective effect of sodium aescinate against hydronephrosis injury in mice after unilateral ureteral obstruction

Effect of remifentanil on the intervertebral disc of rats with intervertebral disc degeneration and its mechanism

Establishment of a multiplex PCR system for rapid detection of sepsis

Inhibitory effect of SAHA on human osteosarcoma 143B cells and its related mechanism

Related Author

Zhang Xing

Leng Weiling

Lei Xiaotian

He Yu

Liang Ziwen

Yan Juhan

Zhao Pengtao

Li Jiacheng

Related Institution

Department of Endocrinology，The First Affiliated Hospital of Army Medical University

The First Affiliated Hospital of Hebei North University

Graduate School of Hebei North University

Department of Pain Treatment，Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University

MyGenostics Inc

⁰