Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells

SU Bo-hua; et al

doi:10.13406/j.cnki.cyxb.2011.01.035

您当前的位置：

首页 >

文章列表页 >

Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells

更新时间：2024-01-17

- Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells
- Journal of Chongqing Medical University Vol. 36, Issue 1, Pages: 11-15(2011)
- 作者机构：
  
  福建医科大学附属口腔医院预防科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.01.035
  CLC： R739.870.22
- Published：2011，
扫描看全文
[1]苏柏华,王锦锋,佘林,郑斐斐,丁林灿,卢友光.CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响[J].重庆医科大学学报,2011,36(01):11-15.

SU Bo-hua, et al. Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells. [J]. Journal of Chongqing Medical University 36(1):11-15(2011)
[1]苏柏华,王锦锋,佘林,郑斐斐,丁林灿,卢友光.CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响[J].重庆医科大学学报,2011,36(01):11-15. DOI： 10.13406/j.cnki.cyxb.2011.01.035.

SU Bo-hua, et al. Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells. [J]. Journal of Chongqing Medical University 36(1):11-15(2011) DOI： 10.13406/j.cnki.cyxb.2011.01.035.

摘要

目的:探讨钙粘素12（Cadherin12

CDH12）对涎腺腺样囊性癌（Salivary adenoid cystic carcinoma

SACC）细胞侵袭和转移能力的影响。方法:以人涎腺腺样囊性癌高转移细胞株（Salivary adenoid cystic carcinoma cell line with high metastatic ability

SACC-M）为研究对象

利用CDH12小分子干扰RNA（Small interfering RNAs

iRNA）对CDH12基因进行沉默

Western blot检测转染前后CDH12的表达变化

Cell Counting Kit-8法检测转染前后细胞的生长速度

体外侵袭实验比较细胞侵袭能力变化

体外迁移运动实验比较细胞运动能力的改变。结果:CDH12 siRNA明显下调CDH12基因的表达。CDH12表达下调后

SACC-M细胞体外侵袭能力显著降低

体外迁移运动能力明显降低。结论:CDH12明显地促进了SACC细胞的体外侵袭和迁移运动

提示CDH12可能在SACC的恶性进展中起着重要作用。

Abstract

Objective:To study the effect of CDH12 on invasion and migration of human salivary adenoid cystic carcinoma cells in vitro.Methods:The highly metastatic human salivary adenoid cystic carcinoma cell line SACC-M was transfected by CDH12 siRNA to silence CDH12 gene.The protein expression of CDH12

before and after siRNA transfection

was examined by Western blot.Cell growth rate was determined with Cell Counting Kit-8.At the same time

the invasive capability and migration capability were evaluated by tumour invasion assay and migration assay

respectively.Results CDH12 siRNA transfection specifically down-regulated the CDH12 protein in SACC-M cells.The downregulation of CDH12 supressed the invasive capability and migration capability of SACC-M cells.Conclusion:CDH12 obviously promotes the invasion and migration of SACC cells in vitro.These results suggest that CDH12 may play an important role in the malignant progression in salivary adenoid cystic carcinoma.

关键词

小分子干扰RNA钙粘素12腺样囊性癌侵袭

Keywords

siRNACDH12adenoid cystic carcinomainvasion

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Proteasome activator subunit 1/2 promotes the proliferation and invasion of endometrial carcinoma cells by inhibiting the expression of cyclin-dependent kinase 15

MCU regulates metastasis of oral squamous cell carcinoma cells by maintaining mitochondrial calcium homeostasis through LETM1

Clinical and biological significance of MYH9 in human hepatocellular carcinoma and its role in cell migration and invasion

Effects of silencing Cyclophilin A by siRNA on proliferation and metastasis in Hep-2 cells

Related Author

Liu Jie

Xie Xingming

Niu Hongxia

Yang Xianzhi

Zhang Hailin

Li Cong

Xiao Zhenghua

Liao Juan

Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Central Laboratory of Yongchuan Hospital Affiliated to Chongqing Medical University

Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University

Department of Obstetrics and Gynecology,Yongchuan Hospital Affiliated to Chongqing Medical University

Department of Stomatology，Tangshan Vocational and Technical College

⁰