Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7

ZHOU Tao-qi; et al

doi:10.13406/j.cnki.cyxb.2011.01.028

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Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7

更新时间：2024-01-17

- Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7
- Journal of Chongqing Medical University Vol. 36, Issue 1, Pages: 54-57(2011)
- 作者机构：
  
  重庆医科大学附属第一医院肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.01.028
  CLC： R737.9
- Published：2011，
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[1]周涛琪,张幸平,程丽,蒋骞,饶明月.5-FU、ADM联合X线外照射对乳腺癌MCF-7细胞Livin表达及凋亡的影响[J].重庆医科大学学报,2011,36(01):54-57.

ZHOU Tao-qi, et al. Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7. [J]. Journal of Chongqing Medical University 36(1):54-57(2011)
[1]周涛琪,张幸平,程丽,蒋骞,饶明月.5-FU、ADM联合X线外照射对乳腺癌MCF-7细胞Livin表达及凋亡的影响[J].重庆医科大学学报,2011,36(01):54-57. DOI： 10.13406/j.cnki.cyxb.2011.01.028.

ZHOU Tao-qi, et al. Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7. [J]. Journal of Chongqing Medical University 36(1):54-57(2011) DOI： 10.13406/j.cnki.cyxb.2011.01.028.

摘要

目的:观察抗肿瘤药物氟尿嘧啶（5-fluorouracil

5-FU）、阿霉素（Adriamycin

ADM）联合X线外照射对人乳腺癌MCF-7细胞Livin基因表达的干扰效应及对细胞凋亡的影响。方法:用MTT法分别测定5-FU、ADM 2种药物作用MCF-7细胞72 h的半数抑制浓度（Half maximal inhibitory concentration of a substanceI

C50）。实验分为空白对照组、单纯照射组、5-FU+照射组、ADM+照射组。通过免疫组化法及RT-PCR法检测各组细胞Livin蛋白及mRNA的表达

同时应用流式细胞术检测细胞的凋亡率。结果:与空白对照组比较

单纯照射组、5-FU+照射组、ADM+照射组的Livin蛋白、mRNA的表达均减少而凋亡率增加（P<0.05）

5-FU+照射组、ADM+照射组的Livin蛋白、mRNA的表达较单纯照射组减少明显

且前2组细胞的凋亡率增加

差异具有统计学意义（P<0.05）。结论:5-FU、ADM联合X线外照射可提高乳腺癌MCF-7细胞的凋亡率

具有放射增敏作用

其作用机制可能与下调Livin基因的表达有关。

Abstract

Objective:To observe 5-FU

ADM combined with X-ray irradiation on interferring gene expression of Livin and apoptosis of human breast cancer cell line MCF-7.Methods:The IC50 of 5-FU

ADM were detected by MTT in the time of 72 h.The experiment had four groups:blank control group

irradiation group

5-FU + irradiation group

ADM + irradiation group.The Immunohistochemistry and RT-PCR were used to evaluate the Livin protein and mRNA expression

the apoptosis rate was analysed by flow cytometry.Results:Compare the blank control group with other groups

the Livin protein and mRNA expression of irradiation group

5-FU + irradiation group

ADM + irradiation group were reduced

and apoptosis rate increased(P<0.05).While the effect of 5-FU

ADM combined with irradiation inhibited Livin protein

mRNA expression significantly compared with the irradiation group

and the apoptosis rate were increased

the differences were statistically significant(P<0.05).Conclusion:5-FU

ADM combined with irradiation can enhance MCF-7 cell apoptosis

with a radiation sensitizing effect

the mechanism may be related to reduce Livin gene expression.

关键词

Livin基因化疗药物RT-PCR免疫组化放射增敏

Keywords

livinchemotherapeuticsRT-PCRimmunohistochemistryradiation sensitive

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