Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism

WANG Yan-li; et al

doi:10.13406/j.cnki.cyxb.2011.01.026

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Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism

更新时间：2024-01-17

- Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism
- Journal of Chongqing Medical University Vol. 36, Issue 1, Pages: 27-30(2011)
- 作者机构：
  
  1. 重庆市第三人民医院消化内科
  2. 重庆医科大学附属第一医院消化外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.01.026
  CLC： R735.2
- Published：2011，
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[1]王艳丽,高青.抑制PI3K/Akt信号通路对胃癌细胞转移和侵袭影响的研究[J].重庆医科大学学报,2011,36(01):27-30.

WANG Yan-li, et al. Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism. [J]. Journal of Chongqing Medical University 36(1):27-30(2011)
[1]王艳丽,高青.抑制PI3K/Akt信号通路对胃癌细胞转移和侵袭影响的研究[J].重庆医科大学学报,2011,36(01):27-30. DOI： 10.13406/j.cnki.cyxb.2011.01.026.

WANG Yan-li, et al. Effects of PI3K/Akt inhibition on the metastasis and invasion of human gastric carcinoma cell SGC-7901 and its mechanism. [J]. Journal of Chongqing Medical University 36(1):27-30(2011) DOI： 10.13406/j.cnki.cyxb.2011.01.026.

摘要

目的:探讨PI3K/Akt信号通路对胃癌细胞转移和侵袭的影响。方法:选用PI3K/Akt信号通路抑制剂LY294002体外作用于胃癌细胞株SGC-7901

Western blot检查总Akt和p-Aktser473蛋白表达

细胞粘附实验观察细胞与基质的粘附力

Transwell法检查细胞侵袭能力

RT-PCR及免疫细胞化学法检查E-cadherin mRNA及蛋白表达。结果:经LY294002处理的SGC-7901

总Akt表达无改变

p-Aktser473蛋白表达下降;细胞与基质的粘附力及侵袭能力减弱;E-cadherin mRNA及蛋白表达水平升高。结论:抑制PI3K/Akt信号通路可减弱胃癌细胞的转移、侵袭能力

其可能机制与上调E-cadherin表达相关。

Abstract

Objective:To investigate the influence of PI3K/Akt on metastasis and invasion of human gastric carcinoma.Methods:Human gastric carcinoma cell line SGC-7901 was cultured with PI3K/Akt signaling pathway inhibitor LY294002 in vitro.The expression of total Akt and p-Aktser473 were detected in protein level by Western blot

the cell-ground substance adhesion was tested by cell adherence assay and the invasion ability was measured with Transwell plate

the expression of E-cadherin in mRNA level and protein level were detected by RT-PCR and S-P immunohistochemistry.Results:The expression of total Akt was constant

but the protein level of p-Aktser473 was down regulated after treatment with LY294002

the adhesion rate and the invasion ability were decreased

the expression of E-cadherin in mRNA and protein were positively regulated.Conclusion:The metastasis and invasion capacity of human gastric carcinoma cell could be decreased by inhibiting PI3K/Akt

perhaps the mechanism is associated with increasing of E-cadherin expression.

关键词

胃癌转移侵袭钙黏蛋白E

Keywords

gastric cancermetastasisinvasione-cadherin

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Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital,Chongqing Medical University

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Department of Gastroenterology,the First Affiliated Hospital,Chongqing Medical University

Department of Digestive Disease,the First Affiliated Hospital,Chongqing Medical University

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