Objective:To construct differential gene expression profile in the DMBA(9

10-Dimethylen-1

2Benzanthracene

DMBA) induced hamster cheek pouch mucosa carcinogenesis process

using bioinformatics analysis methods in screening the genes and signaling pathways which play an important role in carcinogenesis.Methods:The DMBA-induced hamster buccal squamous cell carcinoma model was established;screening criteria of Ratio≥2 and Ratio≤0.5 were used;Agilent whole gene expression profiling microarray was used to detect the differentially expressed genes in hamster cheek pouch mucosa carcinogenesis

and then Gene Ontology for biological function and Pathway analysis were performed with those important virulent genes.Results:A total of 1 943 differentially expressed genes between normal hamster buccal mucosa and cancerous tissue were detected

of which 787 up-regulated and 1 156 down-regulated;GO analysis of differentially expressed genes involved structural elements

transcription regulation

metabolism and so on;pathway analysis found that 27 channels had significant changes

involving a total of 136 different genes.Among them

13 genes

including Ppp3r2

Actn1 and Src involved three or more pathways.Conclusion:During the occurrence of the golden hamster cheek cancer

a total of 1 943 genes were differentially expressed and 27 pathways abnormally changed

among which 13 genes including Ppp3r2

Actn1 and Src lead to changes in cellular pathways and eventually become an important cause of cancer.An in-depth study of those important genes will reveal the molecular mechanism of carcinogenesis of oral mucosa and provide an effective method of targeted therapy.