Biological characteristics of liver tumor in mice implanted with S180 sarcoma

DAI Zhi-kai; et al

doi:10.13406/j.cnki.cyxb.2010.12.033

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Biological characteristics of liver tumor in mice implanted with S180 sarcoma

更新时间：2024-01-17

- Biological characteristics of liver tumor in mice implanted with S180 sarcoma
- Journal of Chongqing Medical University Vol. 35, Issue 12, Pages: 1921-1923(2010)
- 作者机构：
  
  桂林医学院药理学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.12.033
  CLC： R73-352
- Published：2010，
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[1]戴支凯,梁荣感,周巧灵,徐庆,罗伟生.小鼠肝S180肉瘤种植性肿瘤的生物学特性[J].重庆医科大学学报,2010,35(12):1921-1923.

DAI Zhi-kai, et al. Biological characteristics of liver tumor in mice implanted with S180 sarcoma. [J]. Journal of Chongqing Medical University 35(12):1921-1923(2010)
[1]戴支凯,梁荣感,周巧灵,徐庆,罗伟生.小鼠肝S180肉瘤种植性肿瘤的生物学特性[J].重庆医科大学学报,2010,35(12):1921-1923. DOI： 10.13406/j.cnki.cyxb.2010.12.033.

DAI Zhi-kai, et al. Biological characteristics of liver tumor in mice implanted with S180 sarcoma. [J]. Journal of Chongqing Medical University 35(12):1921-1923(2010) DOI： 10.13406/j.cnki.cyxb.2010.12.033.

摘要

目的:建立小鼠肝S180肉瘤种植性肿瘤模型

观察其生物学特性

为肝肿瘤的实验研究提供动物模型平台。方法:采用S180肉瘤瘤块肝内种植的方法建立小鼠肝肿瘤模型

观察S180小鼠免疫器官指数、肿瘤大小、肿瘤转移及荷瘤小鼠寿命。采用HE染色观察肝肿瘤组织的形态结构。免疫组织化学检测肿瘤组织血管内皮生长因子（Vascular endothelial growth factor

VEGF）和肿瘤转移抑制蛋白nm23（nm23）的表达。结果:小鼠肝S180种植性肿瘤模型建立的成功率为100%。S180肝肿瘤小鼠寿命为（22.80±5.87）d。随时间延长

S180肝肿瘤小鼠体重逐渐增加、肿瘤体积逐渐增大、胸腺指数下降

出现腹水及转移的小鼠逐渐增多

无自发性消退现象。肿瘤组织生长旺盛

VEGF呈阳性表达

nm23呈阴性表达。结论:小鼠肝S180肉瘤种植性肿瘤模型是一种良好的肝肿瘤实验动物模型。

Abstract

Objective:To provide an animal model platform of experimental study on liver cancer by establishing a liver tumor model implanted with S180 sarcoma in mice and observing their biological characteristics.Methods:Intrahepatic implantation method was used to establish liver tumor model with S180 tumor piece in mice.Immune organ index

tumor size

tumor metastasis

and life-time of tumor-bearing mice were observed.Tumor tissue morphology was observed by HE staining.Immunohistochemistry was used to detect protein expressions of vascular endothelial growth factor(VEGF) and nm23 in tumor tissue.Results:Modeling achievement ratio of transplantation hepatocarcinoma model with S180 in mice was 100%.The average life expectancy of tumor-bearing mice was 22.80 ± 5.87 days.Body weight and tumor volume increased gradually in a time-dependent manner

but thymus index of the liver tumor mice tended to descend

and the number of mice with ascites and metastasis was gradually increased.Tumor tissue grew strongly;VEGF expression was positive and nm23 was negative in the tissue.Conclusion:The liver tumor model implanted with S180 sarcoma in mice is a good experimental animal model for experimental study on liver tumor.

关键词

小鼠S180肉瘤肝肿瘤模型生物学特性

Keywords

miceS180 sarcomaLiver tumor modelBiological characteristics

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