Establishment of hepatocarcinoma rat modelinduced by diethylmitrosamine discontinuously and pathological study

ZHU Yan-zhi; et al

doi:10.13406/j.cnki.cyxb.2010.12.028

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Establishment of hepatocarcinoma rat modelinduced by diethylmitrosamine discontinuously and pathological study

更新时间：2024-01-17

- Establishment of hepatocarcinoma rat modelinduced by diethylmitrosamine discontinuously and pathological study
- Journal of Chongqing Medical University Vol. 35, Issue 12, Pages: 1843-1846(2010)
- 作者机构：
  
  重庆医科大学附属第一医院肝胆外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.12.028
  CLC： R735.7
- Published：2010，
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[1]朱艳志,孔宪炳,颜朗.间断给药DEN诱导大鼠肝癌模型建立及病理研究[J].重庆医科大学学报,2010,35(12):1843-1846.

ZHU Yan-zhi, et al. Establishment of hepatocarcinoma rat modelinduced by diethylmitrosamine discontinuously and pathological study. [J]. Journal of Chongqing Medical University 35(12):1843-1846(2010)
[1]朱艳志,孔宪炳,颜朗.间断给药DEN诱导大鼠肝癌模型建立及病理研究[J].重庆医科大学学报,2010,35(12):1843-1846. DOI： 10.13406/j.cnki.cyxb.2010.12.028.

ZHU Yan-zhi, et al. Establishment of hepatocarcinoma rat modelinduced by diethylmitrosamine discontinuously and pathological study. [J]. Journal of Chongqing Medical University 35(12):1843-1846(2010) DOI： 10.13406/j.cnki.cyxb.2010.12.028.

摘要

目的:建立间断给药二乙基亚硝胺（Diethylmitrosamine

DEN）诱导SD大鼠肝癌模型

探讨与人类肝细胞癌相似的动物肝癌模型病理形态变化。方法:取体重为170～210 g雄性SD大鼠63只

随机分为实验组（48只）和对照组（15只）

实验组采用0.01%DEN溶液间断喂养SD大鼠诱发肝癌

对照组自由喂养

常规病理HE染色并动态观察肝癌发生的不同时相。结果:设立间断DEN给药诱导SD大鼠肝癌

病理证实大鼠肝癌为肝细胞癌

诱癌率88.9%（32/36）

其末期成功率高

在整个诱癌过程中经历了肝细胞损伤再生→增生-硬化→癌变3个阶段。结论:该造模方法容易、诱癌成功率高、大鼠存活率高。同时

该肝癌模型可以作为研究人类肝癌发生、发展变化病理形态的一种理想动物模型。

Abstract

Objective:To establish hepatocarcinomas rat model by DEN-induced and investigate the pathologic and morphologic changes during hepatocarcinogenesis

and this model was similar to the development of human hepatocellular carcinomas.Methods:sixty-three male spraque-dawley(SD)rats

weighing 170 to 200g were randomly devided into experimental group(48)and control group(15).The intermission in 0.01% DEN was adminstrated to rats and a series histopathology research was employed the hepatic lesions in induced hepatocarcinomas model model.Results:36 rat hepatocellular carcinomas models were successfully induced by the intermisson in DEN through morphologic investigation.The procession of hepatocarcinogenesis in this model included three stages:hepatic toxic lesion

hepatic proliferation/cirrhosis and hepatic carcinogenesis.Conclusion:This improved model was successful and easy

the rate of induced hepatocarcinoma was high and the mortality was low.the hepatocarcinoma model may be an ideal experimental animal model for the clinical medicine study of human hepatocarcinoma.

关键词

二乙基亚硝胺肝癌动物模型病理改变

Keywords

DiethylmitrosamineHepatocarcinomaanimal modelPathological change

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Related Institution

Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

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