Establishment of models of chronic pancreatitis with ethanol and endotoxin

PENG Xiao-hua; et al

doi:10.13406/j.cnki.cyxb.2010.12.009

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Establishment of models of chronic pancreatitis with ethanol and endotoxin

更新时间：2024-01-17

- Establishment of models of chronic pancreatitis with ethanol and endotoxin
- Journal of Chongqing Medical University Vol. 35, Issue 12, Pages: 1851-1853(2010)
- 作者机构：
  
  重庆医科大学附属第一医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.12.009
  CLC： R576
- Published：2010，
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[1]彭晓华,周旭春.酒精联合内毒素诱导慢性胰腺炎模型的建立[J].重庆医科大学学报,2010,35(12):1851-1853.

PENG Xiao-hua, et al. Establishment of models of chronic pancreatitis with ethanol and endotoxin. [J]. Journal of Chongqing Medical University 35(12):1851-1853(2010)
[1]彭晓华,周旭春.酒精联合内毒素诱导慢性胰腺炎模型的建立[J].重庆医科大学学报,2010,35(12):1851-1853. DOI： 10.13406/j.cnki.cyxb.2010.12.009.

PENG Xiao-hua, et al. Establishment of models of chronic pancreatitis with ethanol and endotoxin. [J]. Journal of Chongqing Medical University 35(12):1851-1853(2010) DOI： 10.13406/j.cnki.cyxb.2010.12.009.

摘要

目的:用酒精联合内毒素建立一个新型的慢性胰腺炎（Chronic pancreatitis

CP）的大鼠模型

为研究CP的发病机制提供合适的动物模型。方法:18只1月龄雄性SD大鼠随机分为对照组、酒精组和模型组

每组各6只。饲以25%酒精

12周后反复腹腔注射脂多糖（Lipopolysaccharides

LPS）

建立CP的动物模型。在光镜下观察胰腺组织显微结构变化

用Masson染色法观察胰腺组织胶原的改变

并测定胰腺组织匀浆中的淀粉酶和脂肪酶及血糖浓度。结果:模型组胰腺小叶内可见炎症细胞浸润

胶原纤维较对照组和酒精组明显增多

胰腺组织内淀粉酶和脂肪酶降低（P<0.05）。结论:在长期摄入乙醇的基础上反复腹腔注射LPS可造成CP发生

本模型可以用于CP的研究。

Abstract

Objective:To investigate the pathogenesis of chronic pancreatitis(CP) by establishing a new pancreatitis model in rats suitable for investigation of the processes and mechanisms leading to pancreatic fibrosis.The model was established with ethanol and lipopolysaccharides(LPS

endotoxin).Methods:Eighteen one-month old male SD rats were randomly divided into control group

alcohol group

and model group.After fed with 25% alcohol for twelve weeks

the rats in model group were injected with lipopolysaccharides(LPS).Pancreatic morphology was conducted with haematoxylin-eosin stain

collagen deposition was determined by Masson stain

and the amylase and lipase in pancreatic tissue homogenate were evaluated by colorimetry.Results:Compared with control group

the acinar cells with vacuolization

inflammatory cell infiltration and collagen increasing were observed in the model group.Lipase and amylase were significantly decreased in the model group(P<0.05).Conclusion:Combined with ethanol

LPS can induce chronic pancreatitis.The rat model may be useful for studying the mechanism of chronic pancreatitis.

关键词

酒精内毒素慢性胰腺炎模型

Keywords

EthanolendotoxinChronic pancreatitismodel

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