Effects of paraffin embedding and section-staining on contractin coefficient of spinal cord in rats

LIN Jing-yan; et al

doi:10.13406/j.cnki.cyxb.2010.12.005

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Effects of paraffin embedding and section-staining on contractin coefficient of spinal cord in rats

更新时间：2024-01-17

- Effects of paraffin embedding and section-staining on contractin coefficient of spinal cord in rats
- Journal of Chongqing Medical University Vol. 35, Issue 12, Pages: 1856-1858(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院麻醉科
  2. 川北医学院形态定量研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.12.005
  CLC： R361.2
- Published：2010，
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[1]林菁艳,彭彬,杨正伟,闵苏.石蜡包埋与切片染色对大鼠脊髓腰膨大体积的影响[J].重庆医科大学学报,2010,35(12):1856-1858.

LIN Jing-yan, et al. Effects of paraffin embedding and section-staining on contractin coefficient of spinal cord in rats. [J]. Journal of Chongqing Medical University 35(12):1856-1858(2010)
[1]林菁艳,彭彬,杨正伟,闵苏.石蜡包埋与切片染色对大鼠脊髓腰膨大体积的影响[J].重庆医科大学学报,2010,35(12):1856-1858. DOI： 10.13406/j.cnki.cyxb.2010.12.005.

LIN Jing-yan, et al. Effects of paraffin embedding and section-staining on contractin coefficient of spinal cord in rats. [J]. Journal of Chongqing Medical University 35(12):1856-1858(2010) DOI： 10.13406/j.cnki.cyxb.2010.12.005.

摘要

目的:研究石蜡包埋和切片染色对大鼠脊髓腰膨大皱缩程度的影响。方法:4%多聚甲醛经心脏灌流固定后取大鼠脊髓腰膨大（L4～6）

称重、测量长度并以比重法测定其密度。重量除以密度即可得到固定后体积。将腰膨大均分为6段

随机抽取其中一段用石蜡包埋。垂直于脊髓长轴方向切取厚度为14μm的连续切片。按等距随机原则每15张抽取一张切片

第一张切片在第1～15张中随机抽取。选中的切片HE染色

用VIS体视学设备测定切片面积（A）和染色后厚度（h）。A乘以切片厚度（H=14μm）即可得到包埋后脊髓腰膨大的体积;A乘以h即可得到切片染色后腰膨大的体积。用包埋后和切片染色后腰膨大的体积除以其包埋前的体积

即为石蜡包埋和切片染色后组织的皱缩系数。结果:组织固定后体积为（96.80±13.85）mm2

石蜡包埋后体积为（60.19±19.15）mm2

切片染色后体积为（42.97±16.02）mm2。石蜡包埋后和切片染色后脊髓的皱缩系数分别为62.9%±20.5%和44.8%±16.6%。结论:石蜡包埋和切片染色后脊髓组织明显皱缩。形态学定量研究必需考虑到组织皱缩对结果的影响。

Abstract

Objective:To study the effects of paraffin embedding and section-staining on the contraction of rat spinal cord.Methods:After fixed by 4% paraformaldehyde through heart perfusion

the lumber intumescentia(L4～6) of rats were separated and weighed.Afterwards

their length and density were also measured

thus the volume can be calculated.Then the lumbar intumescentias were divided into 6 sections equally

and one section was randomly selected and embedded by paraffin

and serially sectioned(14 μm).According to the systematic random sampling principles

sections were sampled per 15 sections

and the first section was chosen from the first 15 sections randomly.These sections were stained by hematoxylin and eosin.The area and thickness of each section was measured by VIS stereological system.Thus

the volumes of spinal cord after embedding and section-staining can be calculated.Compared with their initial volumes after fixation

the contration coefficient could be calculated.Results:The volumes of the lumbar intumescentia after fixation

embedding and section-staining were(96.80±13.85) mm2

(60.19±19.15) mm2 and(42.97±16.02) mm2 respectively.And the contration coefficient after embedding and section-staining were 62.9%±20.5% and 44.8%±16.6% respectively.Conclusion:The spinal cord contracts obviously after paraffin embedding and section-staining.These changes should not be ignored when morphometric studies are done.

关键词

石蜡切片染色体视学

Keywords

Paraffin sectionStainingstereology

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