The mechanism of MNU derived rat bladder cancer

CHEN Gang; et al

doi:10.13406/j.cnki.cyxb.2010.11.036

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The mechanism of MNU derived rat bladder cancer

更新时间：2024-01-17

- The mechanism of MNU derived rat bladder cancer
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1665-1667(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿科
  2. 重庆医科大学检验系国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.036
  CLC： R737.14
- Published：2010，
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[1]陈刚,张尧,敬鹏,罗春丽,吴小候.MNU诱导大鼠膀胱癌的机制研究[J].重庆医科大学学报,2010,35(11):1665-1667.

CHEN Gang, et al. The mechanism of MNU derived rat bladder cancer. [J]. Journal of Chongqing Medical University 35(11):1665-1667(2010)
[1]陈刚,张尧,敬鹏,罗春丽,吴小候.MNU诱导大鼠膀胱癌的机制研究[J].重庆医科大学学报,2010,35(11):1665-1667. DOI： 10.13406/j.cnki.cyxb.2010.11.036.

CHEN Gang, et al. The mechanism of MNU derived rat bladder cancer. [J]. Journal of Chongqing Medical University 35(11):1665-1667(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.036.

摘要

目的:研究N-甲基-N-亚硝基脲（N-methyl-N-nitrosourea

MNU）诱导膀胱癌发生的机制。方法:60只SD大鼠随机分成实验组和对照组。实验组用MNU2 mg/次

2周1次定期作膀胱内灌注

共4次。对照组膀胱内灌以生理盐水0.2 ml/次

每2周1次

共4次。第9周未处死大鼠

随即取大鼠膀胱放入-80℃液氮中保存。采用免疫组化检测H-Ras蛋白的表达

RT-PCR检测磷脂酶Cε（PLCε）mRNA的表达。结果:实验组50只SD大鼠36只成瘤

4只大鼠死亡

成瘤率为81.82%。对照组10只大鼠膀胱未见明显变化。免疫组化检测实验组36只成瘤大鼠中32只H-Ras蛋白阳性。对照组大鼠膀胱组织中H-Ras蛋白表达阴性。RT-PCR

检测实验组和对照组大鼠膀胱PLCε的表达。实验组36只成瘤大鼠PLCε阳性

对照组PLCε表达阴性。结论:MNU可诱导大鼠膀胱黏膜发生癌变

其可能机制为引起H-Ras基因突变并且过表达

同时引起PLCεmRNA表达增加。

Abstract

Objective:To investigate the mechanism of N-methyl-N-nitrosourea(MNU) derived bladder cancer.Methods:60 SD Rats were devided into experimental group and control group at random.NMU was used to intravesical perfuse per 2 weeks at the dose of 2 mg for 8 w in experimental group

while saline was used in control group.All rats was sacrificed to get the bladder at the end of 8 w.All bladders were stored in negative 80℃ liquid nitrogen.Ras was detected by immunohistochemistry

and PLCε mRNA was detected by RT-PCR.Results:Tumor appeared in 36 rats out of 50 rats in experimental group. Totally tumor formation rate was 81.82%.While there is no tumor appeared in control group.Ras protein was positive in 32 rats in experimental group by immunohistochemistry

while negative in control group. PCR showed PLCε mRNA was positive in experimental group

while negative in control group.Conclusion:MNU could induce the oncogenesis in bladder.The possible mechanism was MNU make Ras actived and PLCε overexpressed.

关键词

N-甲基-N-亚硝基脲膀胱癌机制H-RasPLCε

Keywords

MNUbladder cancermechanismH-RasPLCε

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