Effect of HSP90β on the leukemic cell line HL60 induced by ATRA

CAI Qian; et al

doi:10.13406/j.cnki.cyxb.2010.11.035

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Effect of HSP90β on the leukemic cell line HL60 induced by ATRA

更新时间：2024-01-17

- Effect of HSP90β on the leukemic cell line HL60 induced by ATRA
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1658-1661(2010)
- 作者机构：
  
  重庆医科大学附属儿童医院血液肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.035
  CLC： R733.7
- Published：2010，
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[1]蔡倩,徐酉华,刘筱梅,郭玉霞.HSP90β在ATRA诱导的白血病细胞株HL60耐药中的作用研究[J].重庆医科大学学报,2010,35(11):1658-1661.

CAI Qian, et al. Effect of HSP90β on the leukemic cell line HL60 induced by ATRA. [J]. Journal of Chongqing Medical University 35(11):1658-1661(2010)
[1]蔡倩,徐酉华,刘筱梅,郭玉霞.HSP90β在ATRA诱导的白血病细胞株HL60耐药中的作用研究[J].重庆医科大学学报,2010,35(11):1658-1661. DOI： 10.13406/j.cnki.cyxb.2010.11.035.

CAI Qian, et al. Effect of HSP90β on the leukemic cell line HL60 induced by ATRA. [J]. Journal of Chongqing Medical University 35(11):1658-1661(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.035.

摘要

目的:通过检测HSP90β基因在白血病细胞株HL60及其耐药株HL60/ATRA中的表达差异

探讨HSP90β基因在ATRA相关多药耐药性发生中的作用。方法:采用ATRA浓度递增及间歇诱导法建立HL60/ATRA耐药细胞株;用MTT法和流式细胞检测技术（Flow cytometry

FCM）

分别检测HL60和HL60/ATRA细胞的增殖及细胞周期;RT-PCR、Western blot法检测HSP90β基因在HL60及HL60/ATRA细胞中的表达;通过MTT法检测HL60和HL60/ATRA细胞对常用化疗药物（VCR、CTX、VP-16、ADM）敏感性。结果:成功构建了白血病细胞耐药株HL60/ATRA;在mRNA和蛋白两个水平

均检测到HSP90β在耐药株HL60/ATRA中的表达较HL60明显增高（P<0.01）;药敏结果显示HL60/ATRA细胞对VCR、CTX、ADM、VP-16的耐药倍数分别为10.74、5.51、4.01、3.24。结论:ATRA能够诱导HL60细胞中HSP90β高表达

经ATRA诱导后的HL60/ATRA细胞对抗癌药物的敏感性显著降低、耐药性增高

这提示HSP90β可能在ATRA相关多药耐药性的发生过程中发挥着重要作用。

Abstract

Objective:To investigate the different expressions of heat shock protein 90β(HSP90β) mRNA and protein in all-trans retinoic acid(ATRA) sensitive leukemia cell line HL-60 and its resistant cell line HL60/ATRA in order to explore the effect of HSP90β on the ATRA multidrug resistance in leukemia.Methods:HL60/ATRA cell line was constructed by increasing ATRA concentration stepwisely and intermittently.The proliferation and sensitivity to anticancer drugs(VCR

CTX

VP-16

ADM) of HL60 and HL60/ATRA were tested by MTT assay.The cell cycle was analyzed using flow cytometry(FCM).The expression of HSP90β in HL60 cells and HL60/ATRA cells was detected with RT-PCR(reverse transcription PCR) and Western blot.Results:HL60/ATRA was successfully constructed.The transcription and protein expression of HSP90β in HL60/ATRA cells was higher than that of HL60 cells statistically(P<0.01).The drug resistant indexes of HL60/ATRA cells to VCR

CTX

VP-16 and ADM increased to 10.74

5.51

4.01 and 3.24

compared with HL60 cells respectively.Conclusion:ATRA could induce the high expression of HSP90 β in HL-60 cells.The sensitivity to anticancer drugs of HL60/ATRA induced by ATRA is decreased

but drug resistance is increased

which suggests that HSP90β may play an important role in the development of ATRA multidrug resistance.

关键词

多药耐药全反式维甲酸HSP90βHL60/ATRA

Keywords

multidrug resistanceall-trans retinoic acidHSP90βHL60/ATRA

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