Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism

YUAN Yuan; et al

doi:10.13406/j.cnki.cyxb.2010.11.034

您当前的位置：

首页 >

文章列表页 >

Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism

更新时间：2024-01-17

- Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1646-1649(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院消化科
  2. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.034
  CLC： R735.2
- Published：2010，
扫描看全文
[1]袁媛,周洲,周静,姜政,黄爱龙,王丕龙.pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究[J].重庆医科大学学报,2010,35(11):1646-1649.

YUAN Yuan, et al. Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism. [J]. Journal of Chongqing Medical University 35(11):1646-1649(2010)
[1]袁媛,周洲,周静,姜政,黄爱龙,王丕龙.pshRNA-MDRla联合TRAIL基因对胃癌细胞株SGC7901/VCR作用及其作用机制的实验研究[J].重庆医科大学学报,2010,35(11):1646-1649. DOI： 10.13406/j.cnki.cyxb.2010.11.034.

YUAN Yuan, et al. Experimental study on the effects of pshRNA-MDRla combined with pBUD-TRAIL in inducing SGC7901/VCR cell apoptosis and proliferation and their mechanism. [J]. Journal of Chongqing Medical University 35(11):1646-1649(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.034.

摘要

目的:探讨pshRNA-MDRla联合pBUD-TRAIL

在体外对胃癌细胞SGC7901/VCR增殖和凋亡的作用及机制

为肿瘤的基因治疗奠定基础。方法:以人胃癌SGC7901/VCR细胞株为研究对象

将pshRNA-MDRla与pBUD-TRAIL共同转染胃癌SGC7901/VCR细胞并在VCR中培养

同时与pshRNA-MDRla组、pBUD-TRAIL组、SGC7901/VCR和空质粒组相比较

以MTT法检测细胞增殖抑制率、以FCM检测细胞凋亡率以及TUNEL法检测细胞凋亡等

观察联合应用组对肿瘤细胞增殖的抑制作用。结果:MTT法显示:对照组（pBUD-TRAIL、pshRNA-MDRla、空质粒和SGC7901/VCR组）胃癌细胞的生长抑制率分别为45%、30%、10%和10%

联合实验组抑制率为70%;FCM检测显示:对照组胃癌细胞的凋亡率分别为25.96%、10.27%、5.96%和4.25%

联合实验组凋亡率为41.23%;与对照组相比

联合实验组抑制率和凋亡率都具有显著性差异;TUNEL检测结果显示:联合组与其他对照组相比较

细胞核明显棕黄色深染

且阳性染色细胞数目明显增多

表明pBUD-TRAIL与pshRNA-MDRla联合作用

凋亡细胞明显增加。结论:pshRNA-MDRla联合pBUD-TRAIL可显著抑制肿瘤细胞的增殖和促进肿瘤细胞的凋亡

推测与pshRNA-MDRla阻止TRAIL外排有关

从而增强TRAIL促进肿瘤细胞凋亡的作用

两者具有协同作用。

Abstract

Objective:To investigate the effects of pshRNA-MDRla combined with TRAIL in treating gastric cancer cell lines SGC7901/VCR and understand their mechanism

in order to lay experimental and theoretical foundation for clinical application of gene tumor therapy.Methods:SGC7901/VCR cells transfected with pBUD-TRAIL and pshRNA-MDRla simultaneously were cultivated with 1mg/L concentration of VCR

and compared with SGC7901/VCR cell transfected with pshRNA-MDRla

pBUD-TRAIL

pBUD groups and SGC7901/VCR group

respectively.The inhibition of cell proliferation was examined by methylthiazolyl tetrazolium(MTT) method

and cell apoptosis was detected by TUNEL method and flow cytometry using PI staining analysis.Results:The inhibition rates in pshRNA-MDRla

pBUD-TRAIL

pBUD and SGC7901/VCR groups were respectively 45%

30%

10%

and 10% by MTT.The apoptosis rates in pshRNA-MDRla

pBUD-TRAIL

pBUD and SGC7901/VCR groups were respectively 25.96%

10.27%

5.96%

and 4.25% by FCM.Compared with control group

the inhibition rate of the proliferation and apoptosis rate of SGC7901/VCR cells in pshRNA-MDRla combinated with TRAIL group was higher(70%

41.23% respectively);Karyopycnosis

nuclear chromosomal condensation and segmentation were observed by TUNEL.pshRNA-MDRla combinated with TRAIL group could significantly induce the apoptosis of tumor.Conclusion:pshRNA-MDRla combined with TRAIL could inhibit the proliferation and induce apoptosis of SGC7901/VCR cells

which could be related with the interfering effects of pshRNA-MDRla by prohibiting TRAIL excretion

so as to intensify the effect of TRAIL in promoting tumor cell apoptosis and have a synergistic action with TRAIL.

关键词

pshRNA-MDRla肿瘤坏死因子相关的凋亡诱导配体增殖凋亡

Keywords

pshRNA-MDRlaTRAILproliferationapoptosis

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells

Primary study on the mechanism of intralesional therapy for hemangioma with glucocorticoid

Related Author

WANG Chuan

et al

姜政

王丕龙

Liu Jie

Xie Xingming

Niu Hongxia

Yang Xianzhi

Related Institution

Department of Gastroenterology,the First Affiliated Hospital,Chongqing Medical University

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Gastroenterology, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Pathology,Molecular Medicine and Cancer Research Cancer,Chongqing Medical University

⁰