Establishment of xenograft tumor model of human neuroblastoma in immunocompetent mice

HE Wen-fei; et al

doi:10.13406/j.cnki.cyxb.2010.11.033

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Establishment of xenograft tumor model of human neuroblastoma in immunocompetent mice

更新时间：2024-01-17

- Establishment of xenograft tumor model of human neuroblastoma in immunocompetent mice
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1627-1629(2010)
- 作者机构：
  
  重庆医科大学附属儿童医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.033
  CLC： R739.4
- Published：2010，
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[1]何文飞,何大维,马超,赵丹,张永波,卞则栋,易钦君,吴鑫.人神经母细胞瘤免疫活性小鼠移植瘤模型的建立[J].重庆医科大学学报,2010,35(11):1627-1629.

HE Wen-fei, et al. Establishment of xenograft tumor model of human neuroblastoma in immunocompetent mice. [J]. Journal of Chongqing Medical University 35(11):1627-1629(2010)
[1]何文飞,何大维,马超,赵丹,张永波,卞则栋,易钦君,吴鑫.人神经母细胞瘤免疫活性小鼠移植瘤模型的建立[J].重庆医科大学学报,2010,35(11):1627-1629. DOI： 10.13406/j.cnki.cyxb.2010.11.033.

HE Wen-fei, et al. Establishment of xenograft tumor model of human neuroblastoma in immunocompetent mice. [J]. Journal of Chongqing Medical University 35(11):1627-1629(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.033.

摘要

目的:探讨建立C57BL/6j小鼠人神经母细胞瘤移植瘤模型可行性。方法:取含1×107个对数生长期SK-N-SH人神经母细胞瘤细胞悬液0.2 ml

接种于C57BL/6j系小鼠右前肢腋窝皮下

观察小鼠成瘤率、相对肿瘤体积（Relative tumor volume

RTV）、相对肿瘤增长速率（K）、相对肿瘤倍增时间（Td）、存活时间及移植瘤病理学情况。结果:C57BL/6j小鼠在3、7 d、3周、4、5、6、7周各年龄段持续成瘤率分别为:100%、100%、90%、70%、60%、0%、0%。成瘤后3～5周内

3周与4周及5周龄接种的肿瘤RTV、K、Td及C57BL/6j存活时间均有差异（P<0.05）。结论:在C57BL/6j小鼠体内成功建立人类异种移植瘤模型。不同年龄段接种的成瘤率有差异

可以根据后期研究目的选择接种建模时间。

Abstract

Objective:To investigate the feasibility of human neuroblastoma xenograft model in C57BL/6j mice.Methods:SK-N-SH cells were collected and condensed as 1×107/ml during the logarithmic growth phase

0.2 ml injected subcutaneously into C57BL/6j mice.The tumor generation rate

relative tumor volume(RTV)

relative growth rate(K)

relative doubling time(Td)

survival time and pathology were tested.Results:The continuing tumor generation rate of C57BL/6j mice was 100%(10/10) in 3 day

100%(10/10) in 7 day

90%(9/10) in 3 week and 70%(7/10) in 4 week

60%(6/10) in 5 week

0%(0/10) in 6 week and 0%(0/10) in 7 week respectively.There were differences in RTV

and survival time(P<0.05) when comparing 3 week C57BL/6j mice with 4 week and 5 week ones. Conclusion:Human neuroblastoma xenograft model is successfully established in the immunocompetent C57BL/6j mice.There is significant difference between the inoculated ages in the tumor generation rate

and time-points of model inoculation can be selected according to later research purposes.

关键词

C57BL/6J小鼠移植瘤动物模型神经母细胞瘤

Keywords

C57BL/6J miceXenograftanimal modelneuroblastoma

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Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

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