The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231

FENG Hong-lei; et al

doi:10.13406/j.cnki.cyxb.2010.11.032

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The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231

更新时间：2024-01-17

- The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1619-1623(2010)
- 作者机构：
  
  重庆医科大学临床检验诊断学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.032
  CLC： R737.9
- Published：2010，
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[1]冯红蕾,王科,孙笑笑,罗进勇,张彦.骨形态发生蛋白9对人乳腺癌MDA-MB-231细胞增殖与凋亡的作用研究[J].重庆医科大学学报,2010,35(11):1619-1623.

FENG Hong-lei, et al. The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231. [J]. Journal of Chongqing Medical University 35(11):1619-1623(2010)
[1]冯红蕾,王科,孙笑笑,罗进勇,张彦.骨形态发生蛋白9对人乳腺癌MDA-MB-231细胞增殖与凋亡的作用研究[J].重庆医科大学学报,2010,35(11):1619-1623. DOI： 10.13406/j.cnki.cyxb.2010.11.032.

FENG Hong-lei, et al. The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231. [J]. Journal of Chongqing Medical University 35(11):1619-1623(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.032.

摘要

目的:研究骨形态发生蛋白9（Bone morphogenetic protein 9

BMP9）对乳腺癌细胞MDA-MB-231增殖、周期及凋亡的影响。方法:半定量RT-PCR法检测高转移性乳腺癌细胞MDA-MB-231和低转移性乳腺癌细胞MCF-7中BMP9的表达

用人BMP9重组腺病毒（AdBMP9）感染MDA-MB-231细胞作为实验组

含GFP的空载腺病毒（AdGFP）感染MDA-MB-231细胞作为对照组

MTT法观察细胞增殖的变化

流式细胞仪分析周期及凋亡的变化。结果:半定量RT-PCR显示

乳腺癌细胞MDA-MB-231中未检测到BMP9 mRNA的表达;MTT结果显示

AdBMP9处理可引起MDA-MB-231细胞增殖抑制

第5 d MDA-MB-231/BMP9组的细胞增殖率（0.8860±0.0532）显著低于MDA-MB-231/GFP组（1.2240±0.1031）（P<0.05）;流式细胞仪分析结果显示

腺病毒感染后第2 d和第3 d

MDA-MB-231/BMP9组细胞周期各相发生变化

G2/M期细胞明显增加

分别为MDA-MB-231/GFP组的3.2倍和2.4倍（P<0.05）;同时BMP9可诱发细胞凋亡

腺病毒感染后第3 d MDA-MB-231/BMP9组细胞凋亡百分率（31.55%±8.26%）明显高于MDA-MB-231/GFP组（3.80%±0.46%）（P<0.05）。结论:提示BMP9可通过阻滞细胞周期进程和诱导细胞凋亡来抑制乳腺癌细胞MDA-MB-231的增殖。

Abstract

Objective:To investigate the effects of bone morphogenetic protein 9 on the proliferation

cell cycle and apoptosis of breast cancer cell line MDA-MB-231.Methods:The expression of BMP9 in high-metastatic cell line MDA-MB-231 and low-metastatic cell line MCF-7 were detected by Semi-quantitative PCR respectively

MDA-MB-231 were transfected with AdBMP9(as experimental groups) and AdGFP(as control groups) respectively

Then the ability of cell proliferation was detected by MTT

cell cycle and apoptosis were detected by flow cytometry.Results:The BMP9 mRNA was not detected in human breast cancer cell line MDA-MB-231.After transfection with AdBMP9

the MDA-MB-231 cells showed an inhibited proliferation

On the fifth day

the growth rate of experimental groups(0.886 0±0.053 2)was significantly lower than that of control groups(1.224 0±0.103 1)(P<0.05);The results of flow cytometry showed that

BMP9 could induce the cell cycle arrest in MDA-MB-231

On the second day and third day after transfection

the cell number of G2/M phase increased significantly

which were 3.2 fold and 2.4 fold that of control groups respectively;It’s also found that after 72h treatment

the apoptosis rate of experimental groups(31.55%±8.26%) was significantly higher than that of control group(3.80%±0.46%)(P<0.05).Conclusion:These data demonstrated that BMP9 could inhibit the proliferation of MDA-MB-231 by blocking cell cycle and inducing apoptosis.

关键词

骨形态发生蛋白9乳腺癌增殖周期凋亡

Keywords

BMP9breast cancerproliferationCell cycleapoptosis

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