Objective:To study the effect of platelet-derived growth factor(PDGF)and c-myc antisense oligodeoxynucleotides(AODN) together on inhibiting the proliferation of vascular smooth muscle cells( VSMC) and the stenosis of transplanted vascular.Method:To select 48 male Zelanian rabbits and were randomly divided into four groups(n=12

in each group):control group

PDGF-AODN group

c-myc-AODN group

PDGF-AODN adding c-myc-AODN group.The left and right external iliac arteries(length 1.0cm) of rabbits were transplanted reciprocally.The transplanted vascular were respectively soaked in lipofiction

PDGF-AODN

c-myc-AODN and PDGF-AODN addingj c-myc-AODN solution about 20 minute

the vascular anastomotic were sutured by 8/0 suture of soaked in AODN solution.Four weeks later

the specimens were harvested for microscopy.The pathological morphology of transplanted vascular were observed under microscope(HE).The intimal thickness and area

stenosis ratio(%) of transplanted vascular were calculate and analysed statistically among group by computer system.The number of positive cells of PDGF’s mRNA in transplanted vascular wall were counted with in situ hybtidzatian histo-cytochemistry and the number of positive cells of c-myc’s protein in transplanted vascular wall were counted by S-P immunochemisty.Result:The intimal thickness and area

stenosis ratio of transplanted vascular

the number of PDGF and c-myc positive cell in PDGF-AODN adding c-myc-AODN group were significantly lower than those in other group(P<0.01)

and that were lower evidently than PDGF-AODN group and c-myc-AODN group.Conclusion:PDGF and c-myc antisense oligodeoxynucleotides together could significantly inhibit the proliferation of VSMC and stenosis of transplanted vascular. The present study lay the foundation for fuetur making use of oligodeoxynucleotides technology to prevent and treat vascular stenosis in transplanted organ.