GOD-POD assay for evaluating insulin resistance of 3T3-L1 adipocytes

ZHU Xiao-yun; et al

doi:10.13406/j.cnki.cyxb.2010.11.017

您当前的位置：

首页 >

文章列表页 >

GOD-POD assay for evaluating insulin resistance of 3T3-L1 adipocytes

更新时间：2024-01-17

- GOD-POD assay for evaluating insulin resistance of 3T3-L1 adipocytes
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1684-1687(2010)
- 作者机构：
  
  中国中医科学院广安门医院糖尿病研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.017
  CLC： R587.1
- Published：2010，
扫描看全文
[1]朱晓云,王朋倩,韩曼,刘喜明.GOD-POD法在3T3-L1脂肪细胞胰岛素抵抗评价中的应用[J].重庆医科大学学报,2010,35(11):1684-1687.

ZHU Xiao-yun, et al. GOD-POD assay for evaluating insulin resistance of 3T3-L1 adipocytes. [J]. Journal of Chongqing Medical University 35(11):1684-1687(2010)
[1]朱晓云,王朋倩,韩曼,刘喜明.GOD-POD法在3T3-L1脂肪细胞胰岛素抵抗评价中的应用[J].重庆医科大学学报,2010,35(11):1684-1687. DOI： 10.13406/j.cnki.cyxb.2010.11.017.

ZHU Xiao-yun, et al. GOD-POD assay for evaluating insulin resistance of 3T3-L1 adipocytes. [J]. Journal of Chongqing Medical University 35(11):1684-1687(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.017.

摘要

目的:通过葡萄糖氧化酶-过氧化物酶（Glucose oxidase-peroxidase

GOD-POD）法检测脂肪细胞葡萄糖消耗

建立简便、经济的脂肪细胞胰岛素敏感性的评价方法。方法:诱导分化成熟的3T3-L1脂肪细胞

分别采用高浓度葡萄糖（25 mmol/L）、地塞米松（Dexamethasone

DEX）（1.0μmol/L）诱导脂肪细胞产生胰岛素抵抗（Insulin resistance

IR）

GOD-POD法测定糖浓度

动态观察诱导过程中细胞糖消耗

并计算胰岛素敏感性指数（Insulin sensitivity index

ISI）;同时应用RT-PCR检测脂联素（Adiponectin）mRNA表达。结果:通过该方法检测

可以反映脂肪细胞对葡萄糖的摄取利用

以胰岛素刺激下较基础状态细胞葡萄糖消耗的增加量

计算出ISI

能够反映细胞对胰岛素的敏感性及抵抗程度。结论:GOD-POD法能够准确测定细胞总体葡萄糖消耗

可用于脂肪细胞糖摄取利用与IR评价

此方法操作简便、经济易行。

Abstract

Objective:To establish a simple and economical method for evaluating the insulin resistance of adipocyte with Glucose oxidase-peroxidase(GOD-POD) assay in vitro.Methods:3T3-L1 cells were induced into mature adipocytes which were stimulated with glucose(25 mmol/L) and DEX(1.0 μmol/L) respectively to induce insulin resistance.Glucose concentration was measured with GOD-POD assay during the induction

the glucose consumption dynamic viewed and the insulin sensitivity index(ISI) calculated.And the mRNA expression of Adiponectin was tested by RT-PCR.Results:The glucose metabolism of adipocytes was detected with this method.The insulin sensitivity and resistance levels of adipocytes were reflected by ISI calculated by the increase of insulin-stimulated glucose consumption.Conclusion:Glucose consumption can be accurately detected with GOD-POD assay which proves to be a simple

easy and economical method to evaluate insulin resistance of adipocytes.

关键词

葡萄糖氧化酶-过氧化物酶胰岛素抵抗3T3-L1脂联素

Keywords

GOD-PODinsulin resistance3T3-L1adiponectin

references

Views

263

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Plasma adiponectin level in patients with wet age-related macular degeneration 

Role of CMHX003，a novel peroxisome proliferator-activated receptor γ selective partial agonist in enhancing insulin sensitivity in 3T3-L1 cells

The role of RNA m⁶A methylation in insulin resistance in adipocytes

Mediating role of TYG-BMI in vitamin D and nonalcoholic fatty liver disease

Association between glucose metabolism disorder and prevalence of thyroid nodule in the elderly in iodine-adequate area

Related Author

Ming Yue

Hu Xiangnan

LI Jibin

Cui Huanhuan

PENG Chuan

Zhang Yuyao

Yu Yang

Chai Shuiqin

Related Institution

Laboratory of Lipid and Glucose Metabolism，The First Affiliated Hospital of Chongqing Medical University

Teaching and Research Section of Medicinal Chemistry，College of Pharmacy，Chongqing Medical University

Teaching and Research Section of Nutrition and Food Hygiene，School of Public Health and Management，Chongqing Medical University

School of Medical Laboratory，Weifang Medical University

Cheeloo College of Medicine，Shandong University

⁰