Ulinastatin inhibits the action of Hypoxia on ENaC-α mRNA and protein expression

DENG Jia; et al

doi:10.13406/j.cnki.cyxb.2010.11.015

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Ulinastatin inhibits the action of Hypoxia on ENaC-α mRNA and protein expression

更新时间：2024-01-17

- Ulinastatin inhibits the action of Hypoxia on ENaC-α mRNA and protein expression
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1623-1626(2010)
- 作者机构：
  
  重庆医科大学附属第二医院呼吸内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.015
  CLC： R563.8
- Published：2010，
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[1]邓嘉,李长毅,王导新.乌司他丁对A549细胞在缺氧条件下ENaC-α表达影响的研究[J].重庆医科大学学报,2010,35(11):1623-1626.

DENG Jia, et al. Ulinastatin inhibits the action of Hypoxia on ENaC-α mRNA and protein expression. [J]. Journal of Chongqing Medical University 35(11):1623-1626(2010)
[1]邓嘉,李长毅,王导新.乌司他丁对A549细胞在缺氧条件下ENaC-α表达影响的研究[J].重庆医科大学学报,2010,35(11):1623-1626. DOI： 10.13406/j.cnki.cyxb.2010.11.015.

DENG Jia, et al. Ulinastatin inhibits the action of Hypoxia on ENaC-α mRNA and protein expression. [J]. Journal of Chongqing Medical University 35(11):1623-1626(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.015.

摘要

目的:研究乌司他丁对缺氧A549细胞ENaC-α mRNA和蛋白表达水平的影响。方法:将A549细胞分组为缺氧对照组和乌司他丁组

分别在3%O2和0%O2的缺氧条件下

用1 250、2 500、5 000 U/ml浓度乌司他丁作用A549细胞

用RT-PCR半定量ENaC-α mRNA表达量

用Western blot半定量ENaC-α蛋白浓度。结果:在不同缺氧程度下

乌司他丁组的ENaC-α mRNA和蛋白表达均高于对照组

呈浓度依赖方式。结论:乌司他丁能够对抗缺氧对ENaC-α表达的抑制作用

提示乌司他丁在急性肺损伤（Acute lung injury

ALI）的治疗过程中具有缓解肺水肿、减轻肺损伤的作用。

Abstract

Objective:To study the effect of Ulinastatin on ENaC-α mRNA and protein of human alveolar epithelial A549 cells which are treated with hypoxia.Methods:A549 cells were divided into two groups

hypoxia group and ulinastatin group.A549 cells were treated with Ulinastatin at the doses of 1 250

2 500 U/ml and 5 000 U/ml for 48 h when cotreated with 3% O2 or 0% O2.The mRNA and protein levels of ENaC-α were determined by semiquantitative RT-PCR and Western blot.Results:Incubation of A549 with 1 250 U/ml

2 500 U/ml and 5 000 U/ml of Ulinastatin for 48 h could significantly upregulate the expression of ENaC-α in a concentration-dependent manner.Conclusion:Ulinastatin could reverse the inhibitory effect of hypoxia on the expression of ENaC-α

which suggests that ulinastatin can promote lung fluid clearance and pulmonary edema reduction.

关键词

乌司他丁上皮钠通道急性肺损伤缺氧

Keywords

ulinastatinEpithelial sodium channelacute lung injuryhypoxia

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