The consequent comparison of two types of hematoxylin in followed-staining of immunohistochemistry

FENG Qing-lin; et al

doi:10.13406/j.cnki.cyxb.2010.11.009

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The consequent comparison of two types of hematoxylin in followed-staining of immunohistochemistry

更新时间：2024-01-17

- The consequent comparison of two types of hematoxylin in followed-staining of immunohistochemistry
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1700-1701(2010)
- 作者机构：
  
  重庆医科大学干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.009
  CLC： R361
- Published：2010，
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[1]冯清林,姜蓉,雷波,彭小华,黄良库.2种苏木精染液在免疫组织化学中复染效果的比较[J].重庆医科大学学报,2010,35(11):1700-1701.

FENG Qing-lin, et al. The consequent comparison of two types of hematoxylin in followed-staining of immunohistochemistry. [J]. Journal of Chongqing Medical University 35(11):1700-1701(2010)
[1]冯清林,姜蓉,雷波,彭小华,黄良库.2种苏木精染液在免疫组织化学中复染效果的比较[J].重庆医科大学学报,2010,35(11):1700-1701. DOI： 10.13406/j.cnki.cyxb.2010.11.009.

FENG Qing-lin, et al. The consequent comparison of two types of hematoxylin in followed-staining of immunohistochemistry. [J]. Journal of Chongqing Medical University 35(11):1700-1701(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.009.

摘要

目的:比较Mayer苏木精和Harris苏木精染液在免疫组织化学复染中的效果

提高免疫组织化学染色质量。方法:采用相同的染色方法分别将两种苏木精染液用于心肌、大脑等多种石蜡切片的SP、SABC免疫组化复染

对比两种染液染色结果。结果:Mayer苏木精复染后的切片细胞核呈深蓝色

细胞浆及周围环境却没有附着色

核质对比清晰。结论:Mayer苏木精染液在免疫组化复染中效果明显优于Harris苏木精。

Abstract

Objective:To compare the consequence ofimmunohistochemistrywith Mayer hematoxylin and Harris hematoxylin followed-staining

and to improve the quality of immunohistochemistry staining.Methods:Two types of hematoxylin were used with the same methodin immunohistochemistry of cardiac muscle and brain

and the results:of followed-staining were compared.Results:The cytoblast withMayer hematoxylin follow-staining was dark blue

but the cytoplasm and the background were not stained

and the difference betweencytoplasm and cytoblast was clear.Conclusion:Mayer hematoxylin followed-staining has better Results than Harris hematoxylin inimmunohistochemistry.

关键词

Mayer苏木精Harris苏木精免疫组织化学复染

Keywords

Mayer hematoxylinHarris hematoxylinimmunohistochemistryFollowed-staining

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