Reseach of epithelial-mesenchymal transitions in bile duct epithelial cells of SD rats induced by LPS

WANG Mai-jian; et al

doi:10.13406/j.cnki.cyxb.2010.11.008

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Reseach of epithelial-mesenchymal transitions in bile duct epithelial cells of SD rats induced by LPS

更新时间：2024-01-17

- Reseach of epithelial-mesenchymal transitions in bile duct epithelial cells of SD rats induced by LPS
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1662-1665(2010)
- 作者机构：
  
  1. 遵义医学院附属医院肝胆外科
  2. 遵义医学院附属医院胃肠外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.008
  CLC： R363
- Published：2010，
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[1]王麦建,杨雪峰,周廷梅,余得刚,江燕,王曙光,赵礼金.LPS刺激SD大鼠胆管上皮细胞表型转化的动物实验研究[J].重庆医科大学学报,2010,35(11):1662-1665.

WANG Mai-jian, et al. Reseach of epithelial-mesenchymal transitions in bile duct epithelial cells of SD rats induced by LPS. [J]. Journal of Chongqing Medical University 35(11):1662-1665(2010)
[1]王麦建,杨雪峰,周廷梅,余得刚,江燕,王曙光,赵礼金.LPS刺激SD大鼠胆管上皮细胞表型转化的动物实验研究[J].重庆医科大学学报,2010,35(11):1662-1665. DOI： 10.13406/j.cnki.cyxb.2010.11.008.

WANG Mai-jian, et al. Reseach of epithelial-mesenchymal transitions in bile duct epithelial cells of SD rats induced by LPS. [J]. Journal of Chongqing Medical University 35(11):1662-1665(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.008.

摘要

目的:探讨上皮标志物上皮性钙粘附蛋白（E-cadherhin）和间叶标志物波形蛋白（Vimentin）在脂多糖（Lipopolysacoharides

LPS）诱导的SD大鼠胆管上皮细胞中的表达及其意义。方法:选用SD大鼠作为研究对象

用LPS（2μg/ml）剌激其胆管

用免疫组化方法检测E-cadherin和Vimentin在胆管细胞中的表达情况。结果:SD大鼠胆管上皮细胞经LPS剌激后

E-cadherin蛋白表达逐渐缺失（P<0.05）

而Vimentin蛋白出现阳性表达（P<0.05）。结论:LPS剌激可使SD大鼠胆管上皮细胞发生上皮细胞间质转型（Epithelial-mesenchymal transitions

EMT）。

Abstract

Objective:To explore the expression and importance of epithelial marker E-cadherhin and mesenehymal marker Vimentin in the bile duct epithelial cells induced by LPS.Methods:Epithelia of intrahepatic bile ducts of SD rats were chosen as research subjects

and put into LPS(2μg/ml). Immunohistochemical technique was applied to detect both the markers of epithelial E-cadherin and mesenehymal Vimentin.Results:E-cadherin gradually reduced(P<0.05)and Vimentin acquired masculine expression after induction(P<0.05).Conclusion:Epithelial-mesenehymal transitions happened in the bile duct epithelial cells inducted by LPS.

关键词

胆管上皮细胞脂多糖转化上皮性钙粘附蛋白波形蛋白上皮细胞间质转型

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