The influence of slidroside to wnt signaling pathway in rat hepatic stellate cells stimulated by acetaldehyde

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The influence of slidroside to wnt signaling pathway in rat hepatic stellate cells stimulated by acetaldehyde

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- The influence of slidroside to wnt signaling pathway in rat hepatic stellate cells stimulated by acetaldehyde
- Journal of Chongqing Medical University Vol. 35, Issue 11, Pages: 1630-1633(2010)
- 作者机构：
  
  中国人民解放军成都军区总医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.11.005
  CLC： R285.5
- Published：2010，
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[1]杨斌,曾维政,蒋明德,刘巧凤,吴晓玲,荣黎.红景天甙对乙醛刺激的大鼠肝星状细胞中Wnt信号通路的影响及其意义探讨[J].重庆医科大学学报,2010,35(11):1630-1633.

YANG Bin, et al. The influence of slidroside to wnt signaling pathway in rat hepatic stellate cells stimulated by acetaldehyde. [J]. Journal of Chongqing Medical University 35(11):1630-1633(2010)
[1]杨斌,曾维政,蒋明德,刘巧凤,吴晓玲,荣黎.红景天甙对乙醛刺激的大鼠肝星状细胞中Wnt信号通路的影响及其意义探讨[J].重庆医科大学学报,2010,35(11):1630-1633. DOI： 10.13406/j.cnki.cyxb.2010.11.005.

YANG Bin, et al. The influence of slidroside to wnt signaling pathway in rat hepatic stellate cells stimulated by acetaldehyde. [J]. Journal of Chongqing Medical University 35(11):1630-1633(2010) DOI： 10.13406/j.cnki.cyxb.2010.11.005.

摘要

目的:通过观察经红景天甙、乙醛处理后的大鼠肝星状细胞（Hepatic stellate cells

HSCs）增殖速度的变化以及细胞内Wnt信号通路中主要下游分子及目的基因mRNA表达和蛋白水平的改变

探讨Wnt信号通路在HSCs中的作用

以及红景天甙对大鼠HSCs Wnt通路和细胞增殖的影响。方法:用红景天甙及乙醛处理体外培养传代的大鼠HSCs[1]

MTT法绘制细胞生长曲线

采用RT-PCR和Western blot的方法分别观察Wnt信号通路中β-catenin和细胞周期蛋白D1（Cell cycle protein D1

CyclinD1）及相关指标的变化。结果:从各组细胞生长曲线的对比发现:红景天甙处理组细胞的增殖速度相对于单用乙醛组明显降低。单用乙醛刺激的大鼠HSCs中

Ⅰ型胶原（Collagen α1）[2]、β-catenin和Cyclin D1的mRNA表达和蛋白合成均明显增强（P<0.05）

红景天组中Cyclin D1和β-catenin的mRNA表达和蛋白合成均出现不同程度的下降（P<0.05）。结论:Wnt信号通路参与了乙醛刺激的大鼠HSCs活化

红景天甙可能是通过降低大鼠HSCs中Wnt信号通路的活性

从而抑制乙醛刺激的大鼠HSCs的增殖。

Abstract

Objective:To explore the role of wnt signaling pathway and the effect of salidroside on wnt pathway and cell proliferation in rat hepatic stellate cells

we detected the diversity of mRNA

protein expression of Wnt signaling pathway downstream molecules and the proliferation of rat hepatic stellate cells treated by acetaldehyde and salidroside.Methods:We treated the rat hepatic stellate cell with salidroside and acetaldehyde

drawn the cell growth curve using the method MTT

β-catenin

Collagenα1 and Cyclin D1 were detected by reverse transcription PCR and Western blot. Results:By comparing the cell growth curve

we found clearly that proliferation rate of HSCs stimulated by acetaldehyde can be inhibited after salidroside treatment.We also found that mRNA and protein synthesis of Collagenα1

Cyclin D1 and β-catenin were enhanced in rat hepatic stellate cell stimulated by acetaldehyde[1]

however the mRNA expression and protein synthesis of Cyclin D1

β-catenin were declined in the cells treated by salidroside than cells stimulated by acetaldehyde only.Conclusion:The classical wnt signaling pathway may be involved in activation of hepatic stellate cells stimulated by acetaldehyde.The salidroside may reduce the proliferation of rat hepatic stellate cell stimulated by acetaldehyde through inhibiting the wnt signaling pathway.

关键词

肝星状细胞Wnt信号通路红景天甙

Keywords

hepatic stellate cellsWnt signaling pathwaysalidroside

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Related Institution

Department of Integration of Traditional Chinese Medicine and Western Medicine,People’s Hospital of Changshou of Chongqing

Department of Integration of Traditional Chinese Medicine and Western Medicine,The First Affiliated Hospital of Chongqing Medical University

成都军区总医院消化内科

Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University

Department of Gastroenterology,the Second Hospital,Chongqing Medical University

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