Effects of 5-FU combined with nimesulide on the expression of VEGF in human esophageal carcinoma Eca-109 (EC109)cell line

LI Min-jie; et al

doi:10.13406/j.cnki.cyxb.2010.10.044

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Effects of 5-FU combined with nimesulide on the expression of VEGF in human esophageal carcinoma Eca-109 (EC109)cell line

更新时间：2024-01-17

- Effects of 5-FU combined with nimesulide on the expression of VEGF in human esophageal carcinoma Eca-109 (EC109)cell line
- Journal of Chongqing Medical University Vol. 35, Issue 10, Pages: 1520-1523(2010)
- 作者机构：
  
  重庆医科大学附属第一医院胸心外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.10.044
  CLC： R735.1
- Published：2010，
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[1]李民杰,向小勇,汪斌,陈焕文.5-氟脲嘧啶联合尼美舒利对人食管癌EC109细胞VEGF表达的研究[J].重庆医科大学学报,2010,35(10):1520-1523.

LI Min-jie, et al. Effects of 5-FU combined with nimesulide on the expression of VEGF in human esophageal carcinoma Eca-109 (EC109)cell line. [J]. Journal of Chongqing Medical University 35(10):1520-1523(2010)
[1]李民杰,向小勇,汪斌,陈焕文.5-氟脲嘧啶联合尼美舒利对人食管癌EC109细胞VEGF表达的研究[J].重庆医科大学学报,2010,35(10):1520-1523. DOI： 10.13406/j.cnki.cyxb.2010.10.044.

LI Min-jie, et al. Effects of 5-FU combined with nimesulide on the expression of VEGF in human esophageal carcinoma Eca-109 (EC109)cell line. [J]. Journal of Chongqing Medical University 35(10):1520-1523(2010) DOI： 10.13406/j.cnki.cyxb.2010.10.044.

摘要

目的:通过观察5-氟脲嘧啶（5-Fluorouracil

5-FU）、尼美舒利（Nimesulide

NIM）单独或联合作用对人食管癌EC109细胞血管内皮生长因子（Vascular endothelial growth factor

VEGF）表达的影响

初步探讨5-FU、NIM抑制食管癌血管生成的机制。方法:培养食管癌EC109细胞

不同浓度5-FU、NIM单独或联合作用24 h后

MTT法检测细胞增殖情况;流式细胞仪检测细胞凋亡;RT-PCR检测VEGF mRNA表达情况;Western blot法检测VEGF蛋白表达。结果:NIM与5-FU联合作用于食管癌EC109细胞株时

其抑制率与凋亡率较两者单独作用时高

且VEGF mRNA及蛋白的表达水平低于两者单独作用时的表达水平。结论:NIM联合5-FU能有效抑制食管癌EC109细胞增殖

其中抑制VEGF表达可能为其机制之一。

Abstract

Objective:To investigate the effects ofNIM(a cyclooxygenase 2 inhibitor)

5-FU

and their combination on the expression of VEGF

in order to explore the possible mechanism. Methods:Nimesulide (100

200

400μmol/ml)and 5-FU(100

200

400μmol/ml) were used.EC109 cell lines were divided into control group

Nimesulide treated group

5-FU treated group

and Nimesulide combined with 5-FU group.The inhibition on the proliferation of EC109 cells respectively treated with Nimesulide

5-FU

and their combination were detected by MTT and their apoptosis rates were detected by FCM.The expression of VEGF mRNA was detected by RT-PCR. The protein expression of VEGF was detected by Western blotting. Results :The inhibition rate and apoptosis rate ofNimesulide combined with 5-FU were significantly higher than the single Nimesulide or 5-FU single treated.Nimesulide combined with 5-FU significantly reduced VEGF mRNA and protein

as compared with the single Nimesulide and 5-Fu.Conclusion:Nimesulide combined with 5-FU can inhibit the EC109 esophageal cancer cell proliferation in vitro

inhibiting VEGF expression may be one of its mechanisms.

关键词

5-氟脲嘧啶尼美舒利食管癌血管内皮生长因子

Keywords

5-fluorouracilNimesulideesophageal carcinomavascular endothelial growth factor

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