Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells

WANG Chuan; et al

doi:10.13406/j.cnki.cyxb.2010.10.043

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Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells

更新时间：2024-01-17

- Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells
- Journal of Chongqing Medical University Vol. 35, Issue 10, Pages: 1516-1519(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院消化内科
  2. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.10.043
  CLC： R735.2
- Published：2010，
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[1]王川,吴莹,姜政,黄爱龙,王丕龙.白黎芦醇联合TRAIL基因对胃癌SGC-7901细胞增殖与抑制作用的实验研究[J].重庆医科大学学报,2010,35(10):1516-1519.

WANG Chuan, et al. Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells. [J]. Journal of Chongqing Medical University 35(10):1516-1519(2010)
[1]王川,吴莹,姜政,黄爱龙,王丕龙.白黎芦醇联合TRAIL基因对胃癌SGC-7901细胞增殖与抑制作用的实验研究[J].重庆医科大学学报,2010,35(10):1516-1519. DOI： 10.13406/j.cnki.cyxb.2010.10.043.

WANG Chuan, et al. Experimental study on the effects and mechanism of resveratrol combined with TRAIL in inducing apoptosis and inhibiting proliferation of SGC-7901 cells. [J]. Journal of Chongqing Medical University 35(10):1516-1519(2010) DOI： 10.13406/j.cnki.cyxb.2010.10.043.

摘要

目的:探讨白黎芦醉（Resveratrol

Res）联合肿瘤坏死因子相关的凋亡诱导配体（Tumor necrosis factor-related apoptosis inducing ligand

TRAIL）在体外对胃癌SGC-7901细胞增殖和凋亡作用及其机制

为将Res作为抗癌药物的临床应用提供理论和实验依据。方法:以人胃癌SGC-7901细胞株为研究对象

将Res与转染了pBUD-TRAIL质粒的胃癌SGC-7901细胞共同培养

以MTT法检测细胞增殖抑制率;以FCM检测细胞凋亡率;以TUNEL法检测细胞凋亡等

观察联合应用对肿瘤细胞的抑制作用。结果:MTT和FCM检测结果显示:TRAIL组肿瘤细胞抑制率为54.28%

凋亡率17.12%

Res联合TRAIL组抑制率和凋亡率均明显提高

分别为70%和22.03%

与对照组相比

具有显著性差异;TUNEL检测结果显示:TRAIL与Res联合组与对照组相比较

细胞核明显棕黄色深染

且阳性染色细胞数目明显增多

表明TRAIL与Res联合作用凋亡细胞明显增加。结论:Res联合TRAIL基因可显著抑制肿瘤细胞的增殖和促进肿瘤细胞的凋亡

说明Res可增强TRAIL促进肿瘤细胞凋亡

两者具有协同作用。

Abstract

Objective:To investigate the effects of resveratrol (Res) combined with TRAIL on gastric cancer and understand its mechanism

in order to lay experimental and theoretical foundation for clinical application of Res tumortherapy. Methods:SGC-7901 cells transfected with pBUD-TRAIL were processed with a 1/2 IC 50 concentration of Res. The inhibition of cell proliferation were examined by methyhhiazdyl tetrazolium(MTT)method

and cell apoptosis were detect by TUNEL method and flow cytometry using PI staining analysis. The findings in SGC-7901 cells transfected with pBUD and those in SGC-7901 cells transfected with pBUD-TRAIL were compared. Results:Compared with control group

the inhibition rate of the proliferation and apoptosis rate of SGC7901 cells in Res combined with TRAIL group were higher (70% vs 54.28%

22.03% vs 17.12%

respectively)by MTT and FCM. Karyopycnosis

nuclear chromosomal condensation and segmentation were observed by TUNEL. Res could significantly induce the apoptosis of tumor combined with TRAIL. Conclusion:Res combined with TRAIL could inhibit the proliferation and induce apoptosis of SGC7901 cell

demonstrating that Res could intensify the effect of TRAIL in promoting tumor cell apoptosis and have a synergistic action with TRAIL.

关键词

白藜芦醇肿瘤坏死因子相关的凋亡诱导配体增殖凋亡

Keywords

resveratrolTRAILproliferationapoptosis

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周静

姜政

王丕龙

Liu Jie

Xie Xingming

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Related Institution

Department of Gastroenterology,the First Affiliated Hospital,Chongqing Medical University

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Gastroenterology, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Pathology,Molecular Medicine and Cancer Research Cancer,Chongqing Medical University

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