Objective:Package of constructed recombinant tumor-selective type I Herpes simplex virus vector plasmid containing UL38 and CMV promoter and GALV.fus gene (HSV-UL38P-GALV.fus、HSV-CMVP-GALV.fus)

GALV.fus gene was replaced by EGFP in control plasmid (HSV-CMVP-EGFP). The three recombinant viruses were named Synco-2、Synco-1 and Baco-1 respectively。Methods: At first the three constructed plasmids were amplificated in coliform bacterium and transfected into Vero cells by using lipofectamine respectively. Then

these three recombinant type I Herpes simplex viruses were propagated in Vero cells and purified by cesium chloride density purification

titrated by TCID50 method. In the end

Total RNA was extracted from the HepG2 cells which were transfected by Synco-1 and Synco-2 and GALV.fus mRNA was detected by RT-PCR. Results:The three recombinant HSV-1 vectors were packaged successfully

which were propagated in Vero cells and purified by cesium chloride density purification

titrated by TCID50 method. The titre of Baco-1、Synco-1 and Synco-2 were 3×10 10 pfu/ml

1×10 11 pfu/ml and 4×10 10 pfu/ml respectively.GALV.fus gene were identified in the infected HepG2 cells. Conclusion:We packed

amplificated and purified Recombinant HSV-I virus successfully and the expression of GALV.fus gene was identified in the infected HepG2 cells by RT-PCR.